Goat Anti-Chicken IgY H&L Antibody - Secondary antibody, for WB,ELISA,CLIA,LF various applications, from Chicken, generated in Goat, specific to Chicken IgY, Goat IgG
Goat Anti-Chicken IgY H&L Antibody - Secondary antibody, for WB,ELISA,CLIA,LF various applications, from Chicken, generated in Goat, specific to Chicken IgY, Goat IgG
Goat Anti-Chicken IgY H&L Antibody - Secondary antibody, for WB,ELISA,CLIA,LF various applications, from Chicken, generated in Goat, specific to Chicken IgY, Goat IgG
ExactAb™, Validated, Carrier Free, Azide Free, High performance, 6.0 mg/ml
Host Species
Goat
Host species
Goat
Application
WB,ELISA,CLIA,LF
Immunogen
Chicken IgY
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description
Goat Anti-Chicken IgY H&L Antibody, Secondary antibody, could be used for WB, ELISA, CLIA, LF, GICA and so on. WB: 1/400 - 1/2000 ELISA : 1/5000 - 1/15000 LF: 0.5-1.0mg/ml
Product Properties
Antibody Type
secondary antibody
Clonality
polyclonal antibodies
Isotype
Goat IgG
SDS-PAGE
150kDa
Purification Method
Antigen affinity purified
Source
Serum
Form
Liquid
Concentration
6.0 mg/ml
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Goat Anti-Chicken IgY H&L Antibody (Ab170784) - ELISA Goat Anti-Chicken IgY H&L Antibody (Ab170784) was tested by direct ELISA. The plate was coated with different amounts of Chicken IgY Antibody (Ab008417). A serial dilution Goat Anti-Chicken IgY H&L Antibody (Ab170784) was then added at a series of different dilution ratios. Rabbit Anti-Goat IgG H&L (HRP) (Ab141534) was used at 1/10000 dilution as the secondary antibody.
Goat Anti-Chicken IgY H&L Antibody (Ab170784) - ELISA Cross-reactivity of Goat Anti-Chicken IgY H&L Antibody (Ab170784) was tested by ELISA. The wells were coated with the indicated IgG standards at 0.5 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Goat Anti-Chicken IgY H&L Antibody was then added at different dilution ratio followed by incubation for 1h. Rabbit Anti-Goat IgG H&L (HRP) (Ab141534) was used at 1/10000 dilution as the secondary antibody.
Goat Anti-Chicken IgY H&L Antibody (Ab170784) - SDS-PAGE 3 μg/lane of Goat Anti-Chicken IgY H&L Antibody (Ab170784) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining. Showing the band at 26.1 kDa (Light Chain) & 53.1 kDa (Heavy Chain), under reducing conditions; 162.9 kDa, under non-reducing conditions.
Application
Application
Dilution info
WB
1/400 - 1/2000
Certificates
Certificate of Analysis(COA)
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