Goat Anti-Human IgG (AF405)

Features and benefits
  • Short Overview:

    pAb; Goat Anti-Human IgG H&L Antibody (AF405); IF, Flow

  • Species reactivity(Reacts with): Human
  • Isotype: IgG
    Application:
  • Flow Cytometry
  • IF/ICC
Item Number
Ab181959
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab181959-10μg (Trial Size)
Apply for free trial size(?)
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10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$19.90
Ab181959-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$39.90
Ab181959-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$99.90

pAb; Goat Anti-Human IgG H&L Antibody (AF405); IF, Flow

Basic Description

Product NameGoat Anti-Human IgG (AF405)
Specifications & PurityExactAb™, Validated, 1.0 mg/mL
Host speciesGoat
ConjugationAlexa Fluor® 405
GradeExactAb™, Validated
Product Description

Application
Flow: 1:200-1:2000
IF: 1:100-1:1000.

Product Properties

IsotypeIgG
Purification Methodantigen affinity chromatography
Concentration1.0 mg/mL
Storage TempProtected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

Goat Anti-Human IgG (AF405) (Ab181959) - IF
IF analysis of EGFR (blue) in A431 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Serclutamab (anti-EGFR) (Ab182867) at 1/1500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (AF405) (Ab181959) at a dilution of 1/500 for 1 hour at room temperature in the dark (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with PI (red). Images were taken on the confocal laser scanning microscope.

Goat Anti-Human IgG (AF405) (Ab181959) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling CD276 (red) with Mirzotamab (anti-CD276) (Ab182952). Goat Anti-Human IgG (AF405) (Ab181959) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Goat Anti-Human IgG (AF405) (Ab181959) - Flow Cytometry
Flow Cytometry analysis of MCF-7 cells labelling IGF1R (red) with Ganitumab (anti-IGF1R) (Ab177931). Goat Anti-Human IgG (AF405) (Ab181959) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Application

ApplicationDilution info
Flow

1/200-1/2000

IF

1/100-1/1000

Certificates

Certificate of Analysis(COA)

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4 results found

Lot NumberCertificate TypeDateItem
ZJ24F0707764Certificate of AnalysisJul 04, 2024 Ab181959
ZJ24F0707763Certificate of AnalysisJul 04, 2024 Ab181959
ZJ24F0707762Certificate of AnalysisJul 04, 2024 Ab181959
ZJ24R0600728Certificate of AnalysisJun 21, 2024 Ab181959

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