Goat Anti-Human IgG Antibody - Secondary antibody, ≥ 95%, high purity, Human IgG1
Specifications & Purity
≥ 95%
Host species
Goat
Conjugation
Unconjugated
Product Description
Purified goat polyclonal antibody, suitable for LFAs,ELISA,CLIA, WB and other immune detection platforms and other suitable application scenarios. Antigen affinity chromatography, purity greater than 95%, buffer system 0.01M PBS (pH7.4), 0.2μm filtration, no preservatives added.
Product Properties
Purification Method
ntigen affinity chromatography
Purity
≥ 95%
Form
liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Images
Goat Anti-Human IgG Antibody (Ab170447) - ELISA Goat Anti-Human IgG Antibody (Ab170447) was tested by ELISA. The plate was coated with different amounts of human IgG (Ab170213) (3-fold started from 1000 ng/mL. Goat Anti-Human IgG Antibody (Ab170447) was then added at a series of different dilution ratios. Rabbit-Anti-Goat IgG H&L HRP (Ab141534) was used at 1/10000 dilution as the secondary antibody.
Goat Anti-Human IgG Antibody (Ab170447) - ELISA Cross-reactivity of Goat Anti-Human IgG Antibody (Ab170447) was tested by ELISA. The wells were coated with the indicated IgG standards at 1 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Goat Anti-Human IgG Antibody was then added at different dilution ratio followed by incubation for 1h. Rabbit-Anti-Goat IgG H&L HRP (Ab141534) was used at 1/10000 dilution as the secondary antibody. For the batch tested, Goat Anti-Human IgG Antibody (Ab170447) showed a cross-reactivity with rabbit IgG when the dilution ratio of Rabbit anti-mouse IgG antibody is less than 1/8000.
Certificates
Certificate of Analysis(COA)
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.