Secondary Antibody; Goat Anti-Human IgG (Biotin); ELISA, WB, IF, Flow
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab175832-100μg (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $19.90 | |
| Ab175832-1mg | 1mg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $119.90 |
Secondary Antibody; Goat Anti-Human IgG (Biotin); ELISA, WB, IF, Flow
| Product Name | Goat Anti-Human IgG (Biotin) - Secondary antibody, specific to Human IgG |
|---|---|
| Synonyms | Goat Anti-Human Antibody (Biotin); Biotin-conjugated Goat Anti-Human Antibody; Goat Anti-Human IgG / Biotin antibody; Goat Anti-Human IgG-Biotin antibody; |
| Specifications & Purity | ExactAb™, Validated, Azide Free, High performance, 1.0 mg/mL |
| Host species | Goat |
| Conjugation | Biotin |
| Grade | Azide Free, ExactAb™, High Performance, Validated |
| Product Description |
Application |
| Purification Method | Antigen affinity purified |
|---|---|
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing |
| Shipped In | Wet ice |
| Stability And Storage | Store at 4°C for one year. Avoid repeated freeze/thaw cycles. Protect from light. |
Goat Anti-Human IgG (Biotin) (Ab175832) - ELISA
Goat Anti-Human IgG (Biotin) (Ab175832) was tested by direct ELISA. The plate was coated with different amounts of Human IgG (Ab170213). A serial dilution of Goat Anti-Human IgG (Biotin) (Ab175832) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.
Goat Anti-Human IgG (Biotin) (Ab175832) - ELISA
Cross-reactivity of Goat Anti-Human IgG (Biotin) (Ab175832) was tested by direct ELISA. The wells were coated with the indicated IgG at 0.5 µg/mL (100 µL/well) and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h. Goat Anti-Human IgG (Biotin) (Ab175832) at 1/10000 dilution was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.
| Application | Dilution info |
|---|---|
| WB | 1/10000-1/200000 |
| IF | 1:1000 |
| Flow Cytometry |
1/200 - 1/1000
|
| ELISA | 1:10000-1:200000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1001381 | Certificate of Analysis | Oct 26, 2023 | Ab175832 |
| ZJ23F1001382 | Certificate of Analysis | Oct 26, 2023 | Ab175832 |
