Secondary Antibody; Goat Anti-Human IgG (Cy5); IF, Flow
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab175833-100μg (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $49.90 | |
| Ab175833-1mg | 1mg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $299.90 |
Secondary Antibody; Goat Anti-Human IgG (Cy5); IF, Flow
| Product Name | Goat Anti-Human IgG (Cy5) - Secondary antibody, specific to Human IgG |
|---|---|
| Synonyms | Goat Anti-Human Antibody (Cy5); Cy5-conjugated Goat Anti-Human Antibody; Goat Anti-Human IgG / Cy5 antibody; Goat Anti-Human IgG-Cy5 antibody; |
| Specifications & Purity | ExactAb™, Validated, Azide Free, 1.0 mg/mL |
| Host species | Goat |
| Conjugation | Cy5 |
| Grade | Azide Free, ExactAb™, Validated |
| Product Description | Application |
| Purification Method | Antigen affinity purified |
|---|---|
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Protected from light,Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Protect from light. |
Goat Anti-Human IgG (Cy5) (Ab175833) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD20 (red) with Rituximab (anti-CD20) (R412001). Goat Anti-Human IgG (Cy5) (Ab175833) at a dilution of 1/100 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
Goat Anti-Human IgG (Cy5) (Ab175833) - IF
IF analysis of EGFR (red) in A549 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Necitumumab (anti-EGFR) (Ab169264) at 2 μg/mL in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (Cy5) (Ab175833) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| IF | 1:1000 |
| Flow |
1/100-1/1000
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1001385 | Certificate of Analysis | Jul 30, 2024 | Ab175833 |
| ZJ23F1001409 | Certificate of Analysis | Jul 30, 2024 | Ab175833 |
