Secondary Antibody; Goat Anti-Human IgG Fc Antibody; WB, CLIA, LF, GICA, EIA; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab137858-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $29.90 | |
| Ab137858-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab137858-5mg | 5mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab137858-10mg | 10mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $339.90 |
Secondary Antibody; Goat Anti-Human IgG Fc Antibody; WB, CLIA, LF, GICA, EIA; Unconjugated
| Product Name | Goat Anti-Human IgG Fc Antibody - Secondary antibody, specific to Human IgG Fc, Goat IgG |
|---|---|
| Synonyms | Goat Anti-Human IgG Fc Antibody; Goat Anti Human IgG Fc Antibody; Anti-Human IgG Fc Antibody; Anti Human IgG Fc Antibody |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Goat |
| Immunogen | Human IgG Fc |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High performance, Validated |
| Product Description | Goat Anti-Human IgG Fc Antibody, Secondary antibody, could be used for WB, CLIA, LF, GICA, EIA and so on. |
| Isotype | Goat IgG |
|---|---|
| SDS-PAGE | 150kDa |
| Purification Method | Antigen affinity chromatography |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Goat Anti-Human IgG Fc Antibody (Ab137858) - ELISA
Goat Anti-Human IgG Fc Antibody (Ab137858) was tested by ELISA. The plate was coated with 0.5 μg/mL Human IgG (Ab170213). Goat Anti-Human IgG Fc Antibody was biotinylated then added at a series of different dilution ratios. Streptavidin-HRP (np156148) was used at 1/40000 dilution for this ELISA.
Goat Anti-Human IgG Fc Antibody (Ab137858) - ELISA
Cross-reactivity of Goat Anti-Human IgG Fc Antibody (Ab137858) was tested by ELISA. The wells were coated with the indicated IgG standards at 0.5 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Goat Anti-Human IgG Fc Antibody was biotinylated then added at different dilution ratio followed by incubation for 1h. Streptavidin-HRP (np156148) was used at 1/40000 dilution for this ELISA.
For the batch tested, Goat Anti-Human IgG Fc Antibody (Ab137858) showed a high cross-reactivity with Rabbit IgG and a low cross-reactivity with Mouse IgG.
| Application | Dilution info |
|---|---|
| CLIA | 0.5-10 µg/mL |
| LF/ GICA | 0.5-2.0 mg/mL |
| EIA | 1/1000 - 1/50000 |
| WB | 1/500-1/10000 |
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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505339 | Certificate of Analysis | May 11, 2024 | Ab137858 |
| ZJ24F0505338 | Certificate of Analysis | May 11, 2024 | Ab137858 |
| ZJ24F0505337 | Certificate of Analysis | May 11, 2024 | Ab137858 |
