Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free!
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$15.90
Ab181948-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$79.90
Ab181948-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
Goat Anti-Mouse IgG H&L (AF405) - Secondary antibody, specific to Mouse IgG, Goat IgG
Specifications & Purity
Validated, 1.0 mg/mL
Host species
Goat
Immunogen
Mouse IgG
Conjugation
Alexa Fluor® 405
Grade
Validated
Product Description
Flow: 1:100-1:2,000 IF: 1:100-1:1,000
Product Properties
Isotype
Goat IgG
Purification Method
Antigen affinity chromatography
Concentration
1.0 mg/mL
Storage Temp
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C. Avoid freeze/thaw cycle. Protect from light.
Images
Goat Anti-Mouse IgG H&L (AF405) (Ab181948) - IF IF analysis of beta Tubulin (blue) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab179922) at 1/1500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF405) (Ab181948) at a dilution of 1/500 for 1 hour at room temperature in the dark (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with PI (red). Images were taken on the confocal laser scanning microscope.
Goat Anti-Mouse IgG H&L (AF405) (Ab181948) - Flow Cytometry Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094902). Goat Anti-Mouse IgG H&L (AF405) (Ab181948) at a dilution of 1/900 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow
1:100-1:2000
IF
1/100-1/1000
Certificates
Certificate of Analysis(COA)
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ZJ24F0606607