Goat Anti-Mouse IgG H&L (AF405) - Secondary antibody, specific to Mouse IgG, Goat IgG

    Application:
  • Flow Cytometry
  • IF/ICC
Features and benefits
  • Host species: Goat
  • Species reactivity(Reacts with): Mouse
  • Isotype: Goat IgG
  • Conjugation: Alexa Fluor® 405
Item Number
Ab181948
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab181948-10μg (Trial Size)
Apply for free trial size(?)
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10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$15.90
Ab181948-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$79.90
Ab181948-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$399.90

pAb; Goat Anti-Mouse IgG H&L Antibody (AF405); IF, Flow

View related series
Goat Anti-Mouse IgG H&L (AF405)

Basic Description

Product NameGoat Anti-Mouse IgG H&L (AF405) - Secondary antibody, specific to Mouse IgG, Goat IgG
SynonymsGoat Anti-Mouse IgG H&L Antibody | Goat Anti Mouse IgG H&L Antibody | Anti-Mouse IgG H&L Antibody | Anti Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Goat Anti-Mouse IgG Antibody | Goat Anti Mouse IgG Antibody | Anti-Mouse Ig
Specifications & PurityValidated, 1.0 mg/mL
Host speciesGoat
ImmunogenMouse IgG
ConjugationAlexa Fluor® 405
GradeValidated
Product Description

Flow: 1:100-1:2,000
IF: 1:100-1:1,000

Product Properties

IsotypeGoat IgG
Purification MethodAntigen affinity chromatography
Concentration1.0 mg/mL
Storage TempProtected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

Goat Anti-Mouse IgG H&L (AF405) (Ab181948) - IF
IF analysis of beta Tubulin (blue) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab179922) at 1/1500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF405) (Ab181948) at a dilution of 1/500 for 1 hour at room temperature in the dark (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with PI (red). Images were taken on the confocal laser scanning microscope.

Goat Anti-Mouse IgG H&L (AF405) (Ab181948) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094902). Goat Anti-Mouse IgG H&L (AF405) (Ab181948) at a dilution of 1/900 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

ApplicationDilution info
Flow Cytometry

1:100-1:2000

IF/ICC

1/100-1/1000

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0606607Certificate of AnalysisJun 06, 2024 Ab181948
ZJ24F0606606Certificate of AnalysisJun 06, 2024 Ab181948
ZJ24F0606605Certificate of AnalysisJun 06, 2024 Ab181948

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