ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot
Host species
Goat
Immunogen
Mouse IgG
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description
Goat Anti-Mouse IgG H&L Antibody, Secondary antibody, could be uesd for WB, CLIA, LF, GICA, EIA and so on. Application WB: 1/500 - 1/10000 CLIA: 0.5µg/mL-10µg/mL LF/ GICA: 0.5mg/mL-2.0mg/mL EIA: 1/1000 - 1/50000
Product Properties
Isotype
Goat IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity chromatography
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Goat Anti-Mouse IgG H&L Antibody (Ab138058) - ELISA Goat Anti-Mouse IgG H&L Antibody (Ab138058) was tested by ELISA. The plate was coated with 1.0 μg/mL mouse IgG (Ab170221). Goat Anti-Mouse IgG H&L Antibody was biotinylated then added at a series of different dilution ratios. Streptavidin-HRP (np156148) was used at 1/10000 dilution for this ELISA.
Goat Anti-Mouse IgG H&L Antibody (Ab138058) - ELISA Cross-reactivity of Goat Anti-Mouse IgG H&L Antibody (Ab138058) was tested by ELISA. The wells were coated with the indicated IgG standards at 1.0 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Goat Anti-Mouse IgG H&L Antibody was biotinylated then added at different dilution ratio followed by incubation for 1h. Streptavidin-HRP (np156148) was used at 1/10000 dilution for this ELISA. For the batch tested, Goat Anti-Mouse IgG H&L Antibody (Ab138058) showed a cross-reactivity with Human IgG when the dilution ratio of Rabbit anti-mouse IgG antibody is less than 1/64000.
Application
Application
Dilution info
WB
1/500-1/1000
CLIA
0.5-10 µg/mL
LF/ GICA
0.5-2.0 mg/mL
EIA
1/1000 - 1/50000
Certificates
Certificate of Analysis(COA)
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