pAb; Goat Anti-Mouse IgG H&L Antibody; WB, CLIA, LF, GICA, EIA; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab138058-1mg | 1mg | In stock | $99.90 | |
| Ab138058-2mg | 2mg | In stock | $149.90 | |
| Ab138058-10mg | 10mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $429.90 |
pAb; Goat Anti-Mouse IgG H&L Antibody; WB, CLIA, LF, GICA, EIA; Unconjugated
| Product Name | Goat Anti-Mouse IgG H&L Antibody - Secondary antibody, specific to Mouse IgG, Goat IgG |
|---|---|
| Synonyms | Goat Anti-Mouse IgG H&L Antibody; Goat Anti Mouse IgG H&L Antibody; Anti-Mouse IgG H&L Antibody; Anti Mouse IgG H&L Antibody; Mouse IgG H&L Antibody; Mouse IgG H&L Antibody; Goat Anti-Mouse IgG Antibody; Goat Anti Mouse IgG Antibody; Anti-Mouse IgG Antibo |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Goat |
| Immunogen | Mouse IgG |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High performance, Validated |
| Product Description | Goat Anti-Mouse IgG H&L Antibody, Secondary antibody, could be uesd for WB, CLIA, LF, GICA, EIA and so on. |
| Isotype | Goat IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity chromatography |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Goat Anti-Mouse IgG H&L Antibody (Ab138058) - ELISA
Goat Anti-Mouse IgG H&L Antibody (Ab138058) was tested by ELISA. The plate was coated with 1.0 μg/mL mouse IgG (Ab170221). Goat Anti-Mouse IgG H&L Antibody was biotinylated then added at a series of different dilution ratios. Streptavidin-HRP (np156148) was used at 1/10000 dilution for this ELISA.
Goat Anti-Mouse IgG H&L Antibody (Ab138058) - ELISA
Cross-reactivity of Goat Anti-Mouse IgG H&L Antibody (Ab138058) was tested by ELISA. The wells were coated with the indicated IgG standards at 1.0 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Goat Anti-Mouse IgG H&L Antibody was biotinylated then added at different dilution ratio followed by incubation for 1h. Streptavidin-HRP (np156148) was used at 1/10000 dilution for this ELISA.
For the batch tested, Goat Anti-Mouse IgG H&L Antibody (Ab138058) showed a cross-reactivity with Human IgG when the dilution ratio of Rabbit anti-mouse IgG antibody is less than 1/64000.
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| CLIA | 0.5-10 µg/mL |
| LF/ GICA | 0.5-2.0 mg/mL |
| EIA | 1/1000 - 1/50000 |
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| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101677 | Certificate of Analysis | Nov 16, 2023 | Ab138058 |
| ZJ23F1101678 | Certificate of Analysis | Nov 16, 2023 | Ab138058 |
