Goat Anti-Mouse IgG H&L (Biotin) - Secondary antibody, specific to Mouse IgG

Features and benefits
  • Short Overview:

    Secondary Antibody; Goat Anti-Mouse IgG H&L (Biotin); WB, ELISA, IF, Flow

  • Species reactivity(Reacts with): Mouse
    Application:
  • ELISA
  • IF/ICC
  • WB
Item Number
Ab156253
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab156253-100μg (Trial Size)
Apply for free trial size(?)
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100μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$19.90
Ab156253-1mg
1mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$99.90

Secondary Antibody; Goat Anti-Mouse IgG H&L (Biotin); WB, ELISA, IF, Flow

View related series
Goat Anti-Mouse

Basic Description

Product NameGoat Anti-Mouse IgG H&L (Biotin) - Secondary antibody, specific to Mouse IgG
SynonymsGoat Anti-Mouse IgG H&L Antibody (Biotin); Anti-Mouse IgG H&L Antibody (Biotin); Mouse IgG H&L Antibody (Biotin); Biotin-conjugated Goat Anti-Mouse IgG (H+L); Goat Anti Mouse IgG Antibody (Biotin); Anti-Mouse IgG Antibody (Biotin); Mouse IgG Antibody (Bio
Specifications & PurityExactAb™, Validated, Azide Free, High performance, 1.0 mg/mL
Host speciesGoat
ImmunogenMouse IgG
Positive ControlELISA: Mouse IgG
ConjugationBiotin
GradeAzide Free, ExactAb™, High Performance, Validated
Product Description

Application
WB: 1:10,000-1:200,000
ELISA: 1:20,000-1:400,000
IF: 1:1000

Product Properties

Purification MethodAntigen affinity purified
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at 2-8°C,Protected from light,Avoid repeated freezing and thawing
Shipped InWet ice
Stability And StorageStore at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) - IF
IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at a dilution of 1/200 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) - ELISA
Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) was tested by direct ELISA. The plate was coated with different amounts of Mouse IgG (Ab170221). A serial dilution of Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

Application

ApplicationDilution info
WB

1/10000-1/200000

IF

1:1000

ELISA

1/20000-1/400000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1001198Certificate of AnalysisJul 30, 2024 Ab156253
ZJ23F1001199Certificate of AnalysisJul 30, 2024 Ab156253

Related Documents

Solution Calculators