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Goat Anti-Mouse IgG H&L (Biotin) - Secondary antibody, specific to Mouse IgG, Goat IgG

    Application:
  • ELISA
  • IF/ICC
  • WB
Features and benefitsView More
  • Host species: Goat
  • Species reactivity(Reacts with): Mouse
  • Isotype: Goat IgG
  • Conjugation: Biotin
Item Number
Ab179002
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab179002-100μg (Trial Size)
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100μg
In stock
$29.90
Ab179002-1mg
1mg
In stock
$119.90

Secondary Antibody; Goat Anti-Mouse IgG H&L (Biotin); WB, ELISA, IF, Flow

Basic Description

Product NameGoat Anti-Mouse IgG H&L (Biotin) - Secondary antibody, specific to Mouse IgG, Goat IgG
SynonymsGoat Anti-Mouse IgG H&L Antibody (Biotin) | Anti-Mouse IgG H&L Antibody (Biotin) | Mouse IgG H&L Antibody (Biotin) | Biotin-conjugated Goat Anti-Mouse IgG (H+L) | Goat Anti Mouse IgG Antibody (Biotin) | Anti-Mouse IgG Antibody (Biotin) | Mouse IgG Antibod
Specifications & PurityExactAb™, High Performance, Validated, Azide Free, 1.0 mg/mL
Host speciesGoat
ImmunogenMouse IgG
ConjugationBiotin
GradeAzide Free, ExactAb™, High Performance, Validated
Product Description

Application
WB: 1:5,000-1:80,000
ELISA: 1:5,000-1:80,000

IF: 1:1,000  

Product Properties

IsotypeGoat IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at 2-8°C,Protected from light,Avoid repeated freezing and thawing
Shipped InWet ice
Stability And StorageStore at 4°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) - IF
IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Streptavidin protein (FITC) (rp155876) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at 1/20000 dilution
SA-HRP: 1:20000

Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 67s

Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094902). Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/300 was used as the secondary antibody. Then incubated with Streptavidin Protein (FITC) (rp155876) at a dilution of 1/1000. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) - ELISA
Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) was tested by direct ELISA. The plate was coated with different amounts of Mouse IgG (Ab170221). A serial dilution of Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

Application

ApplicationDilution info
IF/ICC

1:1000

WB

1/5000-1/80000

ELISA

1:5000 - 1:80000

Certificates

C of A & Other Certificates

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDateItem
ZJ24R1201651Certificate of AnalysisDec 26, 2024 Ab179002
ZJ23F1202021Certificate of AnalysisDec 13, 2023 Ab179002
ZJ23F1202022Certificate of AnalysisDec 13, 2023 Ab179002
ZJ23R1200180Certificate of AnalysisDec 07, 2023 Ab179002

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