Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free!
Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing
Shipped In
Wet ice
Stability And Storage
Store at 4°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.
Images
Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) - IF IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Streptavidin protein (FITC) (rp155876) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at 1/20000 dilution SA-HRP: 1:20000
Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 67s
Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) - Flow Cytometry Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094902). Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/300 was used as the secondary antibody. Then incubated with Streptavidin Protein (FITC) (rp155876) at a dilution of 1/1000. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) - ELISA Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) was tested by direct ELISA. The plate was coated with different amounts of Mouse IgG (Ab170221). A serial dilution of Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.
Application
Application
Dilution info
IF/ICC
1:1000
WB
1/5000-1/80000
ELISA
1:5000 - 1:80000
Certificates
C of A & Other Certificates
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