Goat Anti-Mouse IgG H&L (Cy5) - Secondary antibody, specific to Mouse IgG, Goat IgG

Features and benefits
  • Short Overview:

    Secondary Antibody; Goat Anti-Mouse IgG H&L (Cy5); IF, Flow

  • Species reactivity(Reacts with): Mouse
  • Isotype: Goat IgG
    Application:
  • Flow Cytometry
  • IF/ICC
Item Number
Ab179004
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab179004-100μg (Trial Size)
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100μg
In stock
$29.90
Ab179004-1mg
1mg
In stock
$159.90

Secondary Antibody; Goat Anti-Mouse IgG H&L (Cy5); IF, Flow

View related series
Goat Anti-Mouse

Basic Description

Product NameGoat Anti-Mouse IgG H&L (Cy5) - Secondary antibody, specific to Mouse IgG, Goat IgG
SynonymsGoat Anti-Mouse IgG H&L Antibody (Cy5); Anti-Mouse IgG H&L Antibody (Cy5); Mouse IgG H&L Antibody (Cy5); Cy5-conjugated Goat Anti-Mouse IgG (H+L); Goat Anti Mouse IgG Antibody (Cy5); Anti-Mouse IgG Antibody (Cy5); Mouse IgG Antibody (Cy5); Goat anti-Mouse
Specifications & PurityExactAb™, Validated, Azide Free, 1.0 mg/mL
Host speciesGoat
ImmunogenMouse IgG
ConjugationCy5
GradeAzide Free, ExactAb™, Validated
Product Description

Application
IF: 1:1000
Flow: 1:100-1:2000

Product Properties

IsotypeGoat IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration1.0 mg/mL
Storage TempProtected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20℃ for one year. Store in the dark. Avoid freeze/thaw cycles.

Images

Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) - IF
IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/1600 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094902). Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) at a dilution of 1/900 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) - Flow Cytometry
Flow Cytometry analysis of SK-N-SH cells labelling CD56 (red) with CD56 Mouse mAb (Ab006503). Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) at a dilution of 1/2700 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

ApplicationDilution info
IF

1:1000

Flow Cytometry

1:100-1:2000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1202025Certificate of AnalysisDec 13, 2023 Ab179004
ZJ23F1202026Certificate of AnalysisDec 13, 2023 Ab179004

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