Goat Anti-Mouse IgG H&L (FITC) - Secondary antibody, specific to Mouse IgG

Features and benefits
  • Short Overview:

    Secondary Antibody; Goat Anti-Mouse IgG H&L (FITC); IF, Flow

  • Species reactivity(Reacts with): Mouse
    Application:
  • Flow Cytometry
  • IF/ICC
Item Number
Ab156254
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab156254-100μg (Trial Size)
Apply for free trial size(?)
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100μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$29.90
Ab156254-1mg
1mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$199.90

Secondary Antibody; Goat Anti-Mouse IgG H&L (FITC); IF, Flow

View related series
Goat Anti-Mouse

Basic Description

Product NameGoat Anti-Mouse IgG H&L (FITC) - Secondary antibody, specific to Mouse IgG
SynonymsGoat Anti-Mouse IgG H&L Antibody (FITC); Anti-Mouse IgG H&L Antibody (FITC); Mouse IgG H&L Antibody (FITC); FITC-conjugated Goat Anti-Mouse IgG (H+L); Goat Anti Mouse IgG Antibody (FITC); Anti-Mouse IgG Antibody (FITC); Mouse IgG Antibody (FITC); Goat ant
Specifications & PurityExactAb™, Validated, Azide Free, 1.0 mg/mL
Host speciesGoat
ImmunogenMouse IgG
ConjugationFITC
GradeAzide Free, ExactAb™, Validated
Product Description

Application
IF: 1:1000
Flow: 1:200-1:2000

Product Properties

Purification MethodAntigen affinity purified
FormLiquid
Concentration1.0 mg/mL
Storage TempProtected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Goat Anti-Mouse IgG H&L (FITC) (Ab156254) - IF
IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Goat Anti-Mouse IgG H&L (FITC) (Ab156254) - Flow Cytometry
Flow analysis of beta Actin (red line) in Jurkat cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/5000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.

Application

ApplicationDilution info
IF

1:1000

Flow Cytometry

1/200-1/2000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1001206Certificate of AnalysisJul 30, 2024 Ab156254
ZJ23F1001207Certificate of AnalysisJul 30, 2024 Ab156254

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