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ExactAb™, Validated, Azide Free, High performance, 1.0 mg/mL
Host species
Goat
Immunogen
Mouse IgG
Conjugation
PE
Grade
Azide Free, ExactAb™, High performance, Validated
Product Description
Application IF: 1:1000 Flow: 1:100-1:2500
Product Properties
Isotype
Goat IgG
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing
Shipped In
Wet ice
Stability And Storage
Store at 4℃ for one year. Avoid freeze/thaw cycle. Protect from light.
Images
Goat Anti-Mouse IgG H&L (PE) (Ab179006) - IF IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/1600 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (PE) (Ab179006) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Goat Anti-Mouse IgG H&L (PE) (Ab179006) - Flow Cytometry Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094902). Goat Anti-Mouse IgG H&L (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
IF
1:1000
Flow
1:100-1:2500
Certificates
Certificate of Analysis(COA)
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ZJ24F0102529