Goat Anti-Rabbit IgG H&L (AF647)

    Application:
  • IF/ICC
Features and benefits
  • Host species: Goat
  • Species reactivity(Reacts with): Rabbit
  • Isotype: Goat IgG
  • Conjugation: Alexa Fluor® 647
Item Number
Ab181958
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab181958-10μg (Trial Size)
Apply for free trial size(?)
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10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$19.90
Ab181958-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$39.90
Ab181958-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$99.90

pAb; Goat Anti-Rabbit IgG H&L Antibody (AF647); IF

Basic Description

Product NameGoat Anti-Rabbit IgG H&L (AF647)
SynonymsBCM1 surface antigen | BCM1 | BCM1Leukocyte antigen MEM-102 | BLAST | BLAST1 | BLAST1TCT.1 | B-lymphocyte activation marker BLAST-1 | CD48 antigen (B-cell membrane protein) | CD48 antigen | CD48 molecule | CD48 | hCD48 | mCD48 | MEM-102 | SLAMF2 | Antigen
Specifications & PurityExactAb™, Validated, 1.0 mg/mL
Host speciesGoat
ImmunogenRabbit IgG
ConjugationAlexa Fluor® 647
GradeExactAb™, Validated
Product Description

Application
IF: 1:100-1:1000.

Product Properties

IsotypeGoat IgG
Purification Methodantigen affinity chromatography
Concentration1.0 mg/mL
Storage TempProtected from light,Store at -20°C,Avoid repeated freezing and thawing
Stability And StorageStore at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) - IF
IF analysis of MMP9 (red) in U2OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with MMP9 antibody (Ab115721) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark. beta Tubulin Mouse mAb (Ab179922) (green) was stained with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at 1/1000 dilution. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) - IF
IF analysis of MMP9 (red) in U2OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with MMP9 antibody (Ab115721) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) - IF
IF analysis of Alpha Tubulin (red) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant Alpha Tubulin Antibody (Ab088473) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Application

ApplicationDilution info
IF/ICC

1/100-1/1000

Certificates

Certificate of Analysis(COA)

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4 results found

Lot NumberCertificate TypeDateItem
ZJ24F0707760Certificate of AnalysisJul 03, 2024 Ab181958
ZJ24F0707759Certificate of AnalysisJul 03, 2024 Ab181958
ZJ24F0707761Certificate of AnalysisJul 03, 2024 Ab181958
ZJ24R0600730Certificate of AnalysisJun 21, 2024 Ab181958

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