Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) - Secondary antibody, IgG

Features and benefits
  • Short Overview:

    pAb; Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405); ICC, IF, IHC, Flow, ELISA

  • Species reactivity(Reacts with): Rabbit
  • Isotype: IgG
    Application:
  • ELISA
  • Flow Cytometry
  • IF/ICC
Item Number
Ab138202
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab138202-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$87.90
Ab138202-1ml
1ml
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$327.90

pAb; Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405); ICC, IF, IHC, Flow, ELISA

View related series
Goat Anti-Rabbit

Basic Description

Product NameGoat Anti-Rabbit IgG H&L (Alexa Fluor® 405) - Secondary antibody, IgG
SynonymsGoat Anti Rabbit IgG H&L (Alexa Fluor® 405); Goat Anti-Rabbit IgG H&L-Alexa Fluor® 405; Goat Anti Rabbit IgG H&L-Alexa Fluor® 405; Goat Anti-Rabbit IgG H&L (Alexa Fluor 405); Goat Anti Rabbit IgG H&L (Alexa Fluor 405); Goat Anti-Rabbit IgG H&L-Alexa Fluo
Specifications & PurityExactAb™, Validated, High performance, 1.0 mg/mL
Host speciesGoat
ImmunogenRabbit IgG
ConjugationAlexa Fluor® 405
GradeExactAb™, High Performance, Validated
Product Description

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405), Secondary antibody, could be used for ICC, IF, IHC, Flow, ELISA and so on.

Application:
IF: 1/100 - 1/1000
FLow: 1/500 - 1/1000
ELISA: 1/5000 - 1/20000

Background:
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 150 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective

Product Properties

IsotypeIgG
Purification MethodAntigen Affinity Purified
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Images

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (Ab138202) - ICC/IF
IF analysis of HeLa cells labeling PABPN1 with purified PABPN1 Rabbit mAb (Ab119979) at 1/200 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Actin Mouse mAb (Ab155822) at 1/500 dilution - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) 1/1000. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (Ab138202) was used as the secondary antibody at 1/500 dilution. Images were taken on the confocal laser scanning microscope.

Application

ApplicationDilution info
IF

1/100-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0700537Certificate of AnalysisMar 26, 2024 Ab138202
ZJ23F0700538Certificate of AnalysisMar 26, 2024 Ab138202

Related Documents

FAQs and Articles

FAQ
Antibody structure and isotypes
Flow Cytometry
Flow cytometry analysis
How to choose and use antibodies-primary antibody
How to find the right secondary antibody
Antibody applications and techniques
Flow cytometry protocol
Immunocytochemistry (ICC) protocol
Immunohistochemical staining protocol for frozen samples (IHC-Fr)
RNA dot blot protocol
Immunohistochemistry (IHC) staining antigen retrieval protocol
Caspase Immunofluorescence Staining Protocol
General Protocol for Western Blotting
Western Blotting with Fluorescently Labeled Antibodies

Solution Calculators