Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Images
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Ab138205) - ICC/IF IF analysis of HeLa cells labeling PABPN1 with purified PABPN1 Rabbit mAb (Ab119979) at 1/200 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Actin Mouse mAb (Ab155822) at 1/500 dilution - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) 1/1000. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Ab138205) was used as the secondary antibody at 1/500 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.
Application
Application
Dilution info
IF/ICC
1/100-1/1000
Flow Cytometry
1/500-1/1000
Certificates
Certificate of Analysis(COA)
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ZJ23F0700535