Secondary Antibody; Goat Anti-Rabbit IgG H&L Antibody; WB, ELISA, CLIA, LF, GICA; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab138272-1mg | 1mg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $117.90 | |
| Ab138272-5mg | 5mg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $297.90 | |
| Ab138272-10mg | 10mg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $477.90 |
Secondary Antibody; Goat Anti-Rabbit IgG H&L Antibody; WB, ELISA, CLIA, LF, GICA; Unconjugated
| Product Name | Goat Anti-Rabbit IgG H&L Antibody - Secondary antibody, specific to Rabbit IgG, Goat IgG |
|---|---|
| Synonyms | Goat Anti-Rabbit IgG H&L Antibody; Goat Anti Rabbit IgG H&L Antibody; Anti-Rabbit IgG H&L Antibody; Anti Rabbit IgG H&L Antibody; Goat Anti-Rabbit IgG Antibody; Goat Anti Rabbit IgG Antibody; Anti- Rabbit IgG Antibody; Anti Rabbit IgG Antibody |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, 6.0 mg/mL |
| Host species | Goat |
| Immunogen | Rabbit IgG |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Goat Anti-Rabbit IgG H&L Antibody, Secondary antibody, could be used for WB, ELISA, CLIA, LF, GICA and so on. |
| Isotype | Goat IgG |
|---|---|
| SDS-PAGE | 150kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 6.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Goat Anti-Rabbit IgG H&L Antibody (Ab138272) - ELISA
Goat Anti-Rabbit IgG H&L Antibody (Ab138272) was tested by ELISA. The plate was coated with different amounts of Rabbit IgG (Ab170215) (3-fold started from 333 ng/mL). Goat Anti-Rabbit IgG H&L Antibody was then added at a series of different dilution ratios. Rabbit Anti-Goat IgG H&L (HRP) (Ab141534) was used at 1/10000 dilution as the secondary antibody.
Goat Anti-Rabbit IgG H&L Antibody (Ab138272) - ELISA
Cross-reactivity of Goat Anti-Rabbit IgG H&L Antibody (Ab138272) was tested by ELISA. The wells were coated with the indicated IgG standards at 0.5 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Goat Anti-Rabbit IgG H&L Antibody was then added at different dilution ratio followed by incubation for 1h. Rabbit Anti-Goat IgG H&L (HRP) (Ab141534) was used at 1/10000 dilution as the secondary antibody.
For the batch tested, Goat Anti-Rabbit IgG H&L Antibody (Ab138272) showed a cross-reactivity with Human IgG when the dilution ratio of Goat Anti-Rabbit IgG H&L Antibody is less than 1/8000.
| Application | Dilution info |
|---|---|
| LF/ GICA |
0.5-1.0 mg/mL |
| WB |
1/400 - 1/2000
|
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| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0800779 | Certificate of Analysis | Aug 08, 2023 | Ab138272 |
| ZJ23F0800781 | Certificate of Analysis | Aug 08, 2023 | Ab138272 |
| ZJ23F0800780 | Certificate of Analysis | Aug 08, 2023 | Ab138272 |
