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100μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$18.90
Ab170143-1mg
1mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
ExactAb™, Validated, Azide Free, High performance, 1.0 mg/mL
Host species
Goat
Immunogen
Rabbit IgG
Conjugation
Biotin
Grade
Azide Free, ExactAb™, High Performance, Validated
Product Description
Biotin conjugated anti-rabbit secondary antibody is commonly used for immunohistochemistry, Western blotting, and ELISA. Application WB: 1:10000-1:200000 ELISA: 1:10000-1:100000 IF: 1:1000
Product Properties
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing
Shipped In
Wet ice
Stability And Storage
Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid repeated freeze/thaw cycles. Protect from light.
Images
Goat Anti-Rabbit IgG H&L (Biotin) (Ab170143) - IF IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Antibody (Ab091004) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Biotin) (Ab170143) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (Cy5) (rp169047) at a dilution of 1/2000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.
Goat Anti-Rabbit IgG H&L (Biotin) (Ab170143) - ELISA Goat Anti-Rabbit IgG H&L (Biotin) (Ab170143) was tested by direct ELISA. The plate was coated with 100 ng of Rabbit IgG (Ab170215). A serial dilution of Goat Anti-Rabbit IgG H&L (Biotin) (Ab170143) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.
Application
Application
Dilution info
WB
1/10000-1/200000
IF
1:1000
ELISA
1:10000-1:100000
Certificates
Certificate of Analysis(COA)
Enter Lot Number to search for COA:
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ZJ23F1001399