Secondary Antibody; Goat Anti-Rabbit IgG H&L (Biotin); WB, ELISA, IF
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab176442-100μg (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 100μg | In stock | $19.90 | |
| Ab176442-1mg | 1mg | In stock | $109.90 |
Secondary Antibody; Goat Anti-Rabbit IgG H&L (Biotin); WB, ELISA, IF
| Product Name | Goat Anti-Rabbit IgG H&L (Biotin) - Secondary antibody, specific to Rabbit IgG, Goat IgG |
|---|---|
| Synonyms | Goat Anti-Rabbit IgG H&L Antibody (Biotin); Anti-Rabbit IgG H&L Antibody (Biotin); Rabbit IgG H&L Antibody (Biotin); Biotin-conjugated Goat Anti-Rabbit IgG (H+L); Goat Anti Rabbit IgG Antibody (Biotin); Anti-Rabbit IgG Antibody (Biotin); Rabbit IgG Antibo |
| Specifications & Purity | ExactAb™, Validated, Azide Free, High performance, 1.0 mg/mL |
| Host species | Goat |
| Immunogen | Rabbit IgG |
| Conjugation | Biotin |
| Grade | Azide Free, ExactAb™, High Performance, Validated |
| Product Description | Application ELISA: 1:10,000-1:200,000 |
| Isotype | Goat IgG |
|---|---|
| SDS-PAGE | 150kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing |
| Shipped In | Wet ice |
| Stability And Storage | Store at 4°C for one year. Avoid repeated freeze/thaw cycles. Protect from light. |
Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) - Western Blot
All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) at 1/40000 dilution
SA-HRP:1:20000
Predicted band size: 29 kDa
Observed band size: 31 kDa
Exposure time: 7.0 s
Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) - ELISA
Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) was tested by direct ELISA. The plate was coated with different amounts of Rabbit IgG (Ab170215). A serial dilution of Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/10000 dilution for this ELISA.
| Application | Dilution info |
|---|---|
| WB | 1/10000-1/200000 |
| ELISA | 1:10000-1:200000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101827 | Certificate of Analysis | Nov 24, 2023 | Ab176442 |
| ZJ23F1101828 | Certificate of Analysis | Nov 24, 2023 | Ab176442 |
