Goat Anti-Rabbit IgG H&L (FITC) - Secondary antibody, specific to Rabbit IgG, Goat IgG

    Application:
  • Flow Cytometry
  • IF/ICC
Features and benefits
  • Host species: Goat
  • Species reactivity(Reacts with): Rabbit
  • Isotype: Goat IgG
  • Conjugation: FITC
Item Number
Ab176446
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab176446-100μg (Trial Size)
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100μg
In stock
$19.90
Ab176446-1mg
1mg
In stock
$119.90

Secondary Antibody; Goat Anti-Rabbit IgG H&L (FITC); IF, Flow

View related series
Goat Anti-Rabbit

Basic Description

Product NameGoat Anti-Rabbit IgG H&L (FITC) - Secondary antibody, specific to Rabbit IgG, Goat IgG
SynonymsGoat Anti-Rabbit IgG H&L Antibody (FITC) | Anti-Rabbit IgG H&L Antibody (FITC) | Rabbit IgG H&L Antibody (FITC) | FITC-conjugated Goat Anti-Rabbit IgG (H+L) | Goat Anti Rabbit IgG Antibody (FITC) | Anti-Rabbit IgG Antibody (FITC) | Rabbit IgG Antibody (FI
Specifications & PurityExactAb™, Validated, Azide Free, 1.0 mg/mL
Host speciesGoat
ImmunogenRabbit IgG
ConjugationFITC
GradeAzide Free, ExactAb™, Validated
Product Description

Application
IF: 1:1000
Flow: 1:500-1:2500

Product Properties

IsotypeGoat IgG
Purification MethodAntigen affinity purified
FormLiquid
Concentration1.0 mg/mL
Storage TempProtected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Goat Anti-Rabbit IgG H&L (FITC) (Ab176446) - IF
IF analysis of beta Actin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Antibody (Ab091004) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ab176446) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Goat Anti-Rabbit IgG H&L (FITC) (Ab176446) - IF
IF analysis of COX IV (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ab176446) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Application

ApplicationDilution info
IF/ICC

1:1000

Flow Cytometry

1:500-1:2500

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1101794Certificate of AnalysisNov 24, 2023 Ab176446
ZJ23F1101793Certificate of AnalysisNov 24, 2023 Ab176446

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