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Goat Anti-Rabbit IgG H&L (TRITC) - Secondary antibody, specific to Rabbit IgG, Goat IgG
Specifications & Purity
ExactAb™, Validated, Azide Free, 1.0 mg/mL
Host species
Goat
Immunogen
Rabbit IgG
Conjugation
TRITC
Grade
Azide Free, ExactAb™, Validated
Product Description
Application IF: 1:1000
Product Properties
Isotype
Goat IgG
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.
Images
Goat Anti-Rabbit IgG H&L (TRITC) (Ab176448) - IF IF analysis of COX IV (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (TRITC) (Ab176448) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.
Application
Application
Dilution info
IF/ICC
1:1000
Certificates
Certificate of Analysis(COA)
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