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Hoechst 33342 trihydrochloride - ≥98%(HPLC), high purity , CAS No.875756-97-1
Fluorescent dye for staining DNA
Basic Description Synonyms Q27286402 | Bisbenzimide ethoxide trihydrochloride [MI] | Bis-benzimide H-33342 trihydrochloride | sodiumoctane-1-sulfonate | 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzo[d]imidazole trihydrochloride | P976261J69 | 2'-(4-Ethoxyphenyl Specifications & Purity ≥98%(HPLC) Biochemical and Physiological Mechanisms Hoechst 33342 trihydrochloride stains and binds minor groove of AT-rich regions. Used to quantify DNA in viable cells. Cell permeable fluorescent DNA dye. Storage Temp Store at -20°C,Argon charged Shipped In Ice chest + Ice pads
Mechanisms of Action Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References
Names and Identifiers IUPAC Name 2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole;trihydrochloride INCHI InChI=1S/C27H28N6O.3ClH/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33;;;/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31);3*1H InChi Key JABNPSKWVNCGMX-UHFFFAOYSA-N Canonical SMILES CCOC1=CC=C(C=C1)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C.Cl.Cl.Cl Isomeric SMILES CCOC1=CC=C(C=C1)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C.Cl.Cl.Cl PubChem CID 16760503 Molecular Weight 561.93
Chemical and Physical Properties Solubility Solvent:water, Max Conc. mg/mL: 28.1, Max Conc. mM: 50; Solvent:DMSO, Max Conc. mg/mL: 28.1, Max Conc. mM: 50 Sensitivity light sensitive;Hygroscopic
Safety and Hazards(GHS) Pictogram(s) GHS07 Signal Warning Hazard Statements H315: Causes skin irritation
H319: Causes serious eye irritation
H335: May cause respiratory irritation
Precautionary Statements P261: Avoid breathing dust/fume/gas/mist/vapors/spray.
P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.
P280: Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352: IF ON SKIN: wash with plenty of water.
P304+P340: IF INHALED: Remove person to fresh air and keep comfortable for breathing.
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