mAb (4G3); Mouse anti HA tag Antibody; WB, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab106776-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $36.90 | |
| Ab106776-50μl | 50μl | In stock | $109.90 | |
| Ab106776-100μl | 100μl | In stock | $169.90 | |
| Ab106776-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,099.90 |
mAb (4G3); Mouse anti HA tag Antibody; WB, IF, ICC; Unconjugated
| Product Name | HA tag Mouse mAb - Primary antibody, specific to HA, Mouse IgG1 |
|---|---|
| Synonyms | HA epitope tag antibody; HA1 antibody; HA2 antibody; hemagglutinin antibody; Hemagglutinin HA1 chain antibody; Hemagglutinin HA2 chain antibody; HA Epitope Tag; HA Tag; HA; Hemagglutinin; HA tag antibody; Anti- HA tag antibody |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | HA |
| Immunogen | Synthetic peptide HA-tag. |
| Positive Control | WB: Over-expressed HA-tagged protein in HEK293T cell lysate. IF/ICC: HEK293T cells, transfected with pcDNA3.1-HA-Flag-p62 #PPL00549-2b. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti HA tag Antibody, Monoclonal (4G3), could be used for WB, IF, ICC and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
HA tag Mouse mAb (Ab106776) - Western Blot
All lanes: HA tag Mouse mAb (Ab106776) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 75 kDa
Observed band size: 77 kDa
Exposure time: 30.0 s
HA tag Mouse mAb (Ab106776) - Western Blot
All lanes: HA tag Mouse mAb (Ab106776) at 1/1000 dilution
Samples: Recombinant Human HA-Ubiquitin Protein (rp170435) at 20 ng per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution
Predicted band size: 11 kDa
Observed band size: 10 kDa
Exposure time: 30.0 s
HA tag Mouse mAb (Ab106776) - IF/ICC
IF image of HA tag Mouse mAb (Ab106776) stained HEK293T cells, transfected with pcDNA3.1-HA-Flag-p62. The cells were 4% para-formaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1 h to permeabilise the cells and block non-specific pro-tein-protein interactions. The cells were then incubated with the HA tag Mouse mAb (Ab106776) (1/200) and the antibody Flag-tag pAb(1/200) at 5 µg/mL overnight at 4°C. The secondary anti-body (Red) was Goat anti-Mouse IgG (H+L)-TRITC and the secondary antibody (Green) was Goat anti-Rabbit IgG (H+L)-FITC used at 1/1000 dilution for 1 h. Hoechst33342 was used to stain the cell nuclei (blue).
| Application | Dilution info |
|---|---|
| WB |
1/1000 - 1/2000
|
| IF/ICC | 1/1000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101592 | Certificate of Analysis | Jul 30, 2024 | Ab106776 |
| ZJ23F1101593 | Certificate of Analysis | Jul 30, 2024 | Ab106776 |
| ZJ24F0505917 | Certificate of Analysis | May 21, 2024 | Ab106776 |
