HA tag Mouse mAb - Primary antibody, specific to HA, IgG2b
Synonyms
HA epitope tag antibody | HA1 antibody | HA2 antibody | hemagglutinin antibody | Hemagglutinin HA1 chain antibody | Hemagglutinin HA2 chain antibody | HA Epitope Tag | HA Tag | HA | Hemagglutinin | HA tag antibody | Anti- HA tag antibody
Specifications & Purity
ExactAb™, Azide Free, Carrier Free, Validated, ≥95%(SDS-PAGE), Lot by Lot
Host species
Mouse
Specificity
HA
Immunogen
Influenza virus hemagglutinin protein (AA 98-106).
Positive Control
WB: HEK293 cells transfected with HA tag.
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, Validated
Product Description
Mouse anti HA tag Antibody, Monoclonal antibody(12CA5), could be used for WB, ICC, IF, IP, ELISA and so on. Application WB: Use at an assay dependent concentration. ICC/IF: Use at an assay dependent concentration. IP: Use at an assay dependent concentration. ELISA: Use at an assay dependent concentration. Relevance Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Product Properties
Isotype
IgG2b
SDS-PAGE
26.1 kDa (Light Chain) & 52.3 kDa (Heavy Chain), under reducing conditions; 209.0 kDa, under non-reducing conditions.
Purification Method
Protein G purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at 2-8°C,Freeze prohibited
Shipped In
Wet ice
Stability And Storage
Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon delivery aliquot.
Images
HA tag Mouse mAb (Ab106781) - Western Blot All lanes: HA tag Mouse mAb (Ab106781) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 115 kDa Observed band size: 66, 120 kDa Exposure time: 0.4 s
Application
Application
Dilution info
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Certificates
Certificate of Analysis(COA)
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