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Product Name | HeLa Whole Cell Lysate |
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Grade | Azide Free, Carrier Free |
Product Description | Product Description: The HeLa whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process. HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro. |
Specifications & Purity | Carrier Free, Azide Free, 200μg, Mycoplasma free |
Form | Lyophilized |
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Concentration | 200μg, Mycoplasma free |
Reconstitution | Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white pow |
Storage Temp | Store at -80°C |
Shipped In | Ice chest + Ice pads |
Stability And Storage | Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage. |
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Lot Number | Certificate Type | Date | Item |
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ZJ23F0500180 | Certificate of Analysis | May 31, 2023 | cl155894 |