| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab086831-10μl | 10μl | In stock | $36.90 | |
| Ab086831-50μl | 50μl | In stock | $109.90 | |
| Ab086831-100μl | 100μl | In stock | $169.90 | |
| Ab086831-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,099.90 |
mAb (4C2); Mouse anti His tag Antibody; WB, IF, ICC; Unconjugated
| Product Name | His tag Mouse mAb - Primary antibody, specific to 6X His, Mouse IgG1 |
|---|---|
| Synonyms | 6 His epitope tag antibody | Hexa His tag antibody | HHHHHH epitope tag antibody | HHHHHH tag antibody | His tag antibody | Polyhistidine Tag antibody | Histidine Tag | 6 His epitope tag | 6-His Tag | 6X His Tag | 6X His | 6x-His Tag | 6X-His | H | Hexa H |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, High performance, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | 6X His |
| Immunogen | Synthetic peptide His-tag. |
| Positive Control | WB: His transfected HEK293 cell lysate. IF/ICC: HEK293F cells, transfected with pcDNA3.1-His-TfR-hFC. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Mouse anti His tag Antibody, Monoclonal antibody(4C2), could be used for WB, IF, ICC and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
His tag Mouse mAb (Ab086831) - Western Blot
All lanes: His tag Mouse mAb (Ab086831) at 1/4000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 116 kDa
Observed band size: 47, 53,112 kDa
Exposure time: 21.9 s
His tag Mouse mAb (Ab086831) - IF/ICC
IF image of His tag Mouse mAb (Ab086831) stained HEK293F cells, transfected with pcDNA3.1-TfR-hFC-His. The cells were 4% paraformaldehyde fixed (15 min) and then incubated in 10% normal goat serum for 1h to per-meabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with His tag Mouse mAb (Ab086831) (1/50) overnight at 4°C. The secondary antibody (Green) was Goat anti-Mouse IgG (H+L)-FITC. Hoechst33342 was used to stain the cell nuclei (blue).
His tag Mouse mAb (Ab086831) - IF/ICC
IF image of His tag Mouse mAb (Ab086831) stained HEK293F cells, transfected with pcDNA3.1-His-TfR-hFC. The cells were 4% paraformaldehyde fixed (15 min) and then incubated in 10% normal goat serum for 1h to per-meabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with His tag Mouse mAb (Ab086831) (1/50) overnight at 4°C. The secondary antibody (Green) was Goat anti-Mouse IgG (H+L)-FITC. Hoechst33342 was used to stain the cell nuclei (blue).
| Application | Dilution info |
|---|---|
| IF/ICC | 1/50 - 1/200 "> 1/50 - 1/200 |
| WB |
1/5000 – 1/10000
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404257 | Certificate of Analysis | Apr 09, 2024 | Ab086831 |
| ZJ23F1202138 | Certificate of Analysis | Dec 20, 2023 | Ab086831 |
| ZJ23F1202137 | Certificate of Analysis | Dec 20, 2023 | Ab086831 |
