His tag Mouse mAb - Primary antibody, specific to 6X His, Mouse IgG1
Synonyms
6 His epitope tag antibody; Hexa His tag antibody; HHHHHH epitope tag antibody; HHHHHH tag antibody; His tag antibody; Polyhistidine Tag antibody; Histidine Tag; 6 His epitope tag; 6-His Tag; 6X His Tag ; 6X His; 6x-His Tag; 6X-His; H; Hexa His tag; HHHHH
Specifications & Purity
ExactAb™, Validated, Carrier Free, High performance, 1.0 mg/mL
Host species
Mouse
Specificity
6X His
Immunogen
Synthetic peptide His-tag.
Positive Control
WB: HEK293F whole cell lysate,transfected with pcDNA3.4-Flag-N-RBD-His. IF/ICC: HEK293F cells, transfected with pcDNA3.1-His-TfR-hFC.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, High Performance, Validated
Product Description
Mouse anti His tag Antibody, Monoclonal antibody(4C2), could be used for WB, IF, ICC and so on. Application: WB: 1/5000 - 1/10000 IF/ICC: 1/50 - 1/200 Relevance The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells.
Product Properties
Isotype
Mouse IgG1
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Application
Application
Dilution info
IF/ICC
1/50 - 1/200
WB
1/5000 – 1/10000
Certificates
Certificate of Analysis(COA)
Enter Lot Number to search for COA:
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ZJ24F0404257