mAb (5H5-D6-H3); Mouse anti Human Hsp40 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab108854-10μl | 10μl | In stock | $69.90 | |
| Ab108854-50μl | 50μl | In stock | $199.90 | |
| Ab108854-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab108854-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
mAb (5H5-D6-H3); Mouse anti Human Hsp40 Antibody; WB; Unconjugated
| Product Name | Hsp40 Mouse mAb - Primary antibody, specific to DNAJB1, Mouse IgG1 |
|---|---|
| Synonyms | DnaJ protein homolog 1 | hDj-1 | Heat shock 40 kDa protein 1 | Heat shock protein 40 | HSP40 | Human DnaJ protein 1 | DnaJ (Hsp40) homolog subfmaily B member 1 antibody | DNAJ 1 antibody | DNAJ B1 antibody | DnaJ heat shock protein family (Hsp40) member B |
| Specifications & Purity | ExactAb™, Validated, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | DNAJB1 |
| Immunogen | Recombinant Protein of Hsp40. |
| Positive Control | WB: Jurkat, HeLa, MCF7, Hep G2, A549, HEK293 and NIH/3T3 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description | Mouse anti Human Hsp40 Antibody, Monoclonal antibody (5H5-D6-H3), could be used for WB and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Hsp40 Mouse mAb (Ab108854) - Western Blot
All lanes: Hsp40 Mouse mAb (Ab108854) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 40 kDa
Exposure time: 36.9 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
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| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505908 | Certificate of Analysis | May 21, 2024 | Ab108854 |
| ZJ24F0505909 | Certificate of Analysis | May 21, 2024 | Ab108854 |
| 1. Rauch JN,Gestwicki JE. () Binding of human nucleotide exchange factors to heat shock protein 70 (Hsp70) generates functionally distinct complexes in vitro.. , [PMID:24318877] |
| 2. Hattori, H H and 7 more authors.. (1992) Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells.. Cell structure and function, [PMID:1586970] |
| 3. Raabe, T T and Manley, J L JL.. (1991) A human homologue of the Escherichia coli DnaJ heat-shock protein.. Nucleic acids research, (11): [PMID:1754405] |
| 4. and Ohtsuka, K K.. (1993) Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ.. Biochemical and biophysical research communications, (30): [PMID:8250930] |
| 5. Qian, Y Q YQ, Patel, D D, Hartl, F U FU and McColl, D J DJ.. (1996) Nuclear magnetic resonance solution structure of the human Hsp40 (HDJ-1) J-domain.. Journal of molecular biology, (12): [PMID:8764402] |
| 6. Hata, M M, Okumura, K K, Seto, M M and Ohtsuka, K K.. (1996) Genomic cloning of a human heat shock protein 40 (Hsp40) gene (HSPF1) and its chromosomal localization to 19p13.2.. Genomics, (15): [PMID:8975727] |
| 7. Shi, Y Y, Mosser, D D DD and Morimoto, R I RI.. (1998) Molecular chaperones as HSF1-specific transcriptional repressors.. Genes & development, (1): [PMID:9499401] |
| 8. Melville, M W MW and 5 more authors.. (1999) The cellular inhibitor of the PKR protein kinase, P58(IPK), is an influenza virus-activated co-chaperone that modulates heat shock protein 70 activity.. The Journal of biological chemistry, (5): [PMID:9920933] |
| 9. Grimwood, Jane J and 97 more authors.. (2004) The DNA sequence and biology of human chromosome 19.. Nature, (1): [PMID:15057824] |
| 10. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 11. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
