The HT-29 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process
HT-29 cells are human intestinal epithelial cells which produce the secretory component of Immunoglobulin A (IgA), and carcinoembryonic antigen (CEA). Cells are used for tumourigenicity studies.
Specifications & Purity
Carrier Free, Azide Free, Mycoplasma free
Images
HT-29 Whole Cell Lysate (cl155904) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: K-562 Whole Cell Lysate (cl155898) Lane 2: HEK-293 Whole Cell Lysate (cl155895) Lane 3: A431 Whole Cell Lysate (cl155901) Lane 4: NIH/3T3 Whole Cell Lysate (cl155900) Lane 5: HT-29 Whole Cell Lysate (cl155904)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa Observed band size: 42 kDa
HT-29 Whole Cell Lysate (cl155904) - Western Blot All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: K-562 Whole Cell Lysate (cl155898) Lane 2: HEK-293 Whole Cell Lysate (cl155895) Lane 3: A431 Whole Cell Lysate (cl155901) Lane 4: NIH/3T3 Whole Cell Lysate (cl155900) Lane 5: HT-29 Whole Cell Lysate (cl155904)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa Observed band size: 29 kDa
HT-29 Whole Cell Lysate (cl155904) - Western Blot All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: K-562 Whole Cell Lysate (cl155898) Lane 2: HEK-293 Whole Cell Lysate (cl155895) Lane 3: A431 Whole Cell Lysate (cl155901) Lane 4: NIH/3T3 Whole Cell Lysate (cl155900) Lane 5: HT-29 Whole Cell Lysate (cl155904)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa Observed band size: 58 kDa
Product Specifications
Concentration
Mycoplasma free
Reconstitution
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. Lyophilized contents may appear as either a translucent film or a white po
Storage Temp
Store at -80°C
Shipped In
Ice chest + Ice pads
Stability And Storage
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
Certificates
Certificate of Analysis(COA)
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ZJ23F0800809