Hydroxyproline ((2S,4R)-4-hydroxyproline, Hyp) is a nonproteinogenic amino acid formed by the enzyme prolyl-4-hydroxylase, which catalizes the post-translational modification of polypeptide proline residues following protein synthesis. In animals, hydroxyproline is found almost entirely in collagen, which contains approximately 12-14% hydroxyproline by mass (the fibrilous protein elastin also contains small quantities of hydroxyproline). Hydroxyproline in tissue hydrolysates is thus a direct measure of the amount of collagen present. Collagen is the most abundant protein in mammals and many diseases are believed to affect collagen turnover, including tumor invasion and metastasis, rheumatoid arthritis, cardiopulmonary fibrosis and muscular dystrophy.
Preparation instructions
Suitable for the estimation of hydroxyproline concentration in tissue homogenates (i.e. muscle, liver, lung, etc.) and human or animal biological fluids (serum, urine).
Principle
The classical assay for determination of Hyp is based upon oxidation of Hyp to a pyrrole intermediate, followed by reaction with Ehrlich′s reagent dissolved in concentrated perchloric acid (HClO4). Perchloric acid is a hazardous material that is b
1.J K Wenderott, Carmen G Flesher, Nicki A Baker, Christopher K Neeley, Oliver A Varban, Carey N Lumeng, Lutfiyya N Muhammad, Chen Yeh, Peter F Green, Robert W O'Rourke. (2020-11-26) Elucidating nanoscale mechanical properties of diabetic human adipose tissue using atomic force microscopy.. Scientific reports, 10 ((1)):( 20423-20423 ). [PMID:33235234]
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