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I-A/I-E Rat mAb (AF647)

  • ExactAb™
  • Validated
  • 0.5 mg/mL
Features and benefits
  • Short Overview:

    mAb (M5/114); Rat anti Mouse I-A/I-E Antibody (AF647); Flow

  • Species reactivity(Reacts with): Mouse
  • Isotype: Rat IgG2b
    Application:
  • Flow Cytometry
Item Number
Ab190267
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab190267-10μg (Trial Size)
Apply for free trial size(?)
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10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$47.90
Ab190267-25μg
25μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$95.90
Ab190267-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$219.90

mAb (M5/114); Rat anti Mouse I-A/I-E Antibody (AF647); Flow

Basic Description

Product NameI-A/I-E Rat mAb (AF647)
Specifications & PurityExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological MechanismsBinds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides
Host speciesRat
SpecificityI-A/I-E
ImmunogenActivated C57BL/6 mouse spleen cells
Positive ControlFlow: C57BL/6 mouse splenocytes.
ConjugationAlexa Fluor® 647
GradeExactAb™, Validated

Product Properties

IsotypeRat IgG2b
Light Chain Typekappa
Purification MethodProtein G purified
Concentration0.5 mg/mL
Storage TempStore at 2-8°C,Protected from light,Freeze prohibited
Shipped InWet ice
Stability And StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

I-A/I-E Rat mAb (AF647) (Ab190267) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with I-A/I-E Rat mAb (AF647) (Ab190267) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF647) (Ab190071).

I-A/I-E Rat mAb (AF647) (Ab190267) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with I-A/I-E Rat mAb (AF647) (Ab190267) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (AF647) (Ab190071). Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets

Application

ApplicationDilution info
Flow

≤0.1 μg/test

Certificates

Certificate of Analysis(COA)

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Related Documents

Solution Calculators