| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab190267-10μg (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $47.90 | |
| Ab190267-25μg | 25μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $95.90 | |
| Ab190267-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $219.90 |
mAb (M5/114); Rat anti Mouse I-A/I-E Antibody (AF647); Flow
| Product Name | I-A/I-E Rat mAb (AF647) |
|---|---|
| Synonyms | IAb | Ia2 | Ia-2 | Abeta | H-2Ab | H2-Ab | Rmcs1 | I-Abeta | Eb | Ia4 | H2Eb | Ia-4 | H-2Eb | E-alpha-f | H-2 class II histocompatibility antigen, E-K alpha chain | H-2 class II histocompatibility antigen, E-U alpha chain | H-2E | H2-Ea | H-2Ea | H2-Ea-ps |
| Specifications & Purity | ExactAb™, Validated, Ex:650nm, Em:668nm, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides |
| Host species | Rat |
| Specificity | I-A/I-E |
| Immunogen | Activated C57BL/6 mouse spleen cells |
| Positive Control | Flow: C57BL/6 mouse splenocytes. |
| Conjugation | Alexa Fluor® 647 |
| Grade | Em:668nm, Ex:650nm, ExactAb™, Validated |
| Isotype | Rat IgG2b |
|---|---|
| Light Chain Type | kappa |
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Freeze prohibited |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
I-A/I-E Rat mAb (AF647) (Ab190267) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with I-A/I-E Rat mAb (AF647) (Ab190267) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF647) (Ab190071).
I-A/I-E Rat mAb (AF647) (Ab190267) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with I-A/I-E Rat mAb (AF647) (Ab190267) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (AF647) (Ab190071). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤0.1 μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.1 μg per million cells in 100 µL staining volume. |
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