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I-A/I-E Rat mAb - Primary antibody, specific to I-A/I-E, Rat IgG2b

Azide Free
Carrier Free
ExactAb™
Validated
≥95%(SDS-PAGE)
Lot by Lot

Features and benefits

Short Overview:
mAb (M5/114); Rat anti Mouse I-A/I-E Antibody; in vivo MHC II blockade, Functional assays, ICC, IF, WB, IP, Flow; Unconjugated
Species reactivity(Reacts with): Mouse
Isotype: Rat IgG2b

Application:

Animal Model
Blocking
Flow Cytometry
Functional Assay
IF/ICC
IP
WB

Item Number

Ab115281

Grouped product items
SKU	Size	Availability	Price
Ab115281-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$19.90
Ab115281-50μg	50μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$59.90
Ab115281-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$79.90
Ab115281-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$429.90

mAb (M5/114); Rat anti Mouse I-A/I-E Antibody; in vivo MHC II blockade, Functional assays, ICC, IF, WB, IP, Flow; Unconjugated

View related series

Accession#: P18468 Accession#:P14483 Gene ID:14961 Gene ID:14969 H2-Ab1 H2-Eb1 I-A/I-E MHC Class II

Basic Description

Product Name	I-A/I-E Rat mAb - Primary antibody, specific to I-A/I-E, Rat IgG2b
Specifications & Purity	ExactAb™, Validated, Carrier Free, Azide Free, ≥95%(SDS-PAGE), Lot by Lot
Host species	Rat
Specificity	I-A/I-E
Immunogen	Activated C57BL/6 mouse spleen cells
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, Validated
Product Description	Rat anti Mouse I-A/I-E Antibody, Monoclonal (M5/114), could be used for in vivo MHC II blockade, Functional assays, ICC, IF, WB, IP, Flow and so on. Application: in vivo MHC II blockade: Use at an assay dependent concentration. Functional assays: Use at an assay dependent concentration. ICC/IF: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. IP: Use at an assay dependent concentration. Flow: Use at an assay dependent concentration. Protein Function Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Product Properties

Isotype	Rat IgG2b
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein G purified
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at 2-8°C,Freeze prohibited
Shipped In	Wet ice
Stability And Storage	Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon delivery aliquot.

Images

I-A/I-E Rat mAb (Ab115281) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling I-A/I-E with I-A/I-E Rat mAb (Ab115281) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

I-A/I-E Rat mAb (Ab115281) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling I-A/I-E (red) with I-A/I-E Rat mAb (Ab115281) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
WB	Use at an assay dependent concentration.
Functional Assays	Use at an assay dependent concentration.
IP	Use at an assay dependent concentration.
ICC/IF	Use at an assay dependent concentration.
Flow	Use at an assay dependent concentration.
in vivo MHC II blockade	Use at an assay dependent concentration

Certificates

Certificate of Analysis(COA)

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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0707965	Certificate of Analysis	Jul 09, 2024	Ab115281
ZJ24F0707966	Certificate of Analysis	Jul 09, 2024	Ab115281
ZJ24F0707967	Certificate of Analysis	Jul 09, 2024	Ab115281

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