pAb; Rabbit anti Human ICAD Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab109205-10μl | 10μl | In stock | $59.90 | |
| Ab109205-20μl | 20μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $89.90 | |
| Ab109205-50μl | 50μl | In stock | $179.90 | |
| Ab109205-100μl | 100μl | In stock | $279.90 | |
| Ab109205-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
pAb; Rabbit anti Human ICAD Antibody; WB, IHC; Unconjugated
| Product Name | ICAD Antibody - Primary antibody, specific to ICAD, Rabbit IgG |
|---|---|
| Synonyms | A330085O09Rik antibody; Caspase activated deoxyribonuclease inhibitor short form antibody; DFF 1 antibody; DFF 45 antibody; DFF alpha antibody; DFF-45 antibody; DFF1 antibody; DFF35 antibody; DFF45 antibody; DFFA antibody; Dffa DNA fragmentation factor, a |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, High performance, Lot by Lot |
| Host species | Rabbit |
| Specificity | ICAD |
| Immunogen | Recombinant ICAD expressed in E.coli (AA 51-305). |
| Positive Control | WB: Jurkat, A431, Hep G2, K562 and MCF7 cell lysates. IHC: Human breast cancer and kidney tissues. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, High performance, Validated |
| Product Description | Rabbit anti Human ICAD Antibody, Polyclonal, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified + Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
ICAD Antibody (Ab109205) - Western Blot
All lanes: ICAD Antibody (Ab109205) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 41 kDa
Exposure time: 10.0 s
ICAD Antibody (Ab109205) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL ICAD Antibody (Ab109205)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ICAD Antibody (Ab109205) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 µg/mL ICAD Antibody (Ab109205)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-20 µg/mL |
| WB |
0.01-2 µg/mL
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303639 | Certificate of Analysis | Mar 22, 2024 | Ab109205 |
| ZJ24F0303638 | Certificate of Analysis | Mar 22, 2024 | Ab109205 |
| ZJ24F0303637 | Certificate of Analysis | Mar 22, 2024 | Ab109205 |
| 1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 2. Liu, X X, Zou, H H, Slaughter, C C and Wang, X X.. (1997) DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis.. Cell, (18): [PMID:9108473] |
| 3. Gu, J J and 5 more authors.. (1999) Functional interaction of DFF35 and DFF45 with caspase-activated DNA fragmentation nuclease DFF40.. The Journal of biological chemistry, (23): [PMID:10409614] |
| 4. Oh, J J JJ, Grosshans, D R DR, Wong, S G SG and Slamon, D J DJ.. (1999) Identification of differentially expressed genes associated with HER-2/neu overexpression in human breast cancer cells.. Nucleic acids research, (15): [PMID:10497265] |
| 5. Zhou, P P, Lugovskoy, A A AA, McCarty, J S JS, Li, P P and Wagner, G G.. (2001) Solution structure of DFF40 and DFF45 N-terminal domain complex and mutual chaperone activity of DFF40 and DFF45.. Proceedings of the National Academy of Sciences of the United States of America, (22): [PMID:11371636] |
| 6. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 7. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243] |
| 8. Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25): [PMID:17525332] |
| 9. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 10. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 11. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 12. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 13. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
