mAb (C10); Mouse anti Human IL-18 Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab110010-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $56.90 | |
| Ab110010-20μl | 20μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $79.90 | |
| Ab110010-50μl | 50μl | In stock | $159.90 | |
| Ab110010-100μl | 100μl | In stock | $249.90 | |
| Ab110010-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,799.90 |
mAb (C10); Mouse anti Human IL-18 Antibody; WB, IHC; Unconjugated
| Product Name | IL-18 Mouse mAb - Primary antibody, specific to IL18, Mouse IgG1 |
|---|---|
| Synonyms | Iboctadekin antibody; IFN gamma inducing factor antibody; IFN-gamma-inducing factor antibody; IGIF antibody; IL 1 gamma antibody; IL 18 antibody; IL 1g antibody; IL-1 gamma antibody; IL-18 antibody; IL1 gamma antibody; IL18 antibody; IL18 protein antibody |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot |
| Host species | Mouse |
| Specificity | IL18 |
| Immunogen | Recombinant IL18 expressed in E.coli (AA 94-193) |
| Positive Control | WB: HeLa, A431 and THP-1 cell lysates. IHC: Human kidney, spleen tissues. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human IL-18 Antibody, Monoclonal (C10), could be used for WB, IHC and so on. Application: WB: 0.5-2µg/mL IHC: 5-20µg/mL Protein Function Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A + Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
IL-18 Mouse mAb (Ab110010) - Western Blot
All lanes: IL-18 Mouse mAb (Ab110010) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 20.7 s
IL-18 Mouse mAb (Ab110010) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL IL-18 Mouse mAb (Ab110010)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
IL-18 Mouse mAb (Ab110010) - IHC
DAB staining on IHC
Sample: Human Spleen Tissue
Primary Ab: 20 µg/mL IL-18 Mouse mAb (Ab110010)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
IL-18 Mouse mAb (Ab110010) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL IL-18 Mouse mAb (Ab110010)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
IL-18 Mouse mAb (Ab110010) - IHC
DAB staining on IHC
Sample: Human Spleen Tissue
Primary Ab: 20 µg/mL IL-18 Mouse mAb (Ab110010)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-20 µg/mL |
| WB | 0.5-2µg/mL |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101829 | Certificate of Analysis | Nov 27, 2023 | Ab110010 |
| ZJ23F1101826 | Certificate of Analysis | Nov 27, 2023 | Ab110010 |
| 1. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811] |
| 2. Ushio, S S and 15 more authors.. (1996) Cloning of the cDNA for human IFN-gamma-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:8666798] |
| 3. Tominaga, K K and 7 more authors.. (2000) IL-12 synergizes with IL-18 or IL-1beta for IFN-gamma production from human T cells.. International immunology, [PMID:10653850] |
| 4. Kato, Zenichiro Z and 14 more authors.. (2003) The structure and binding mode of interleukin-18.. Nature structural biology, [PMID:14528293] |
| 5. Gaggero, Alessia A and 7 more authors.. (2004) A novel isoform of pro-interleukin-18 expressed in ovarian tumors is resistant to caspase-1 and -4 processing.. Oncogene, (30): [PMID:15326478] |
| 6. Jourdan, Tony T and 13 more authors.. (2013) Activation of the Nlrp3 inflammasome in infiltrating macrophages by endocannabinoids mediates beta cell loss in type 2 diabetes.. Nature medicine, [PMID:23955712] |
| 7. Wei, Hui H and 6 more authors.. (2014) Structural basis for the specific recognition of IL-18 by its alpha receptor.. FEBS letters, (3): [PMID:25261253] |
| 8. Tsutsumi, Naotaka N and 12 more authors.. (2014) The structural basis for receptor recognition of human interleukin-18.. Nature communications, (15): [PMID:25500532] |
