Purity>95% (SDS-PAGE&SEC); Endotoxin Level < 0.01EU/μg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab177902-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $109.90 | |
| Ab177902-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $519.90 | |
| Ab177902-5mg | 5mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,399.90 | |
| Ab177902-10mg | 10mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,239.90 |
Purity>95% (SDS-PAGE&SEC); Endotoxin Level < 0.01EU/μg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
| Product Name | Imalumab (anti-MIF) - Primary antibody, specific to MIF, >95%, high purity, Human IgG1 |
|---|---|
| Synonyms | GIF antibody; GLIF antibody; Glycosylation inhibiting factor antibody; Glycosylation-inhibiting factor antibody; L-dopachrome isomerase antibody; L-dopachrome tautomerase antibody; Macrophage migration inhibitory factor (glycosylation-inhibiting factor) a |
| Specifications & Purity | Carrier Free, Recombinant, ExactAb™, Low Endotoxin, Azide Free, Validated, ≥95%(SDS-PAGE&SEC), Lot by Lot |
| Specificity | MIF |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated |
| Action Type | INHIBITOR |
| Mechanism of action | INHIBITOR of Macrophage migration inhibitory factor inhibitor |
| Product Description |
Imalumab (anti-MIF) is a recombinant, human IgG1 monoclonal antibody that targets macrophage inhibitory factor (MIF). Imalumab (anti-MIF) can be used for the research of ovarian carcinoma, recurrent malignant ascites and cancer. |
| Isotype | Human IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 27.9 kDa (Light Chain) & 51.8 kDa (Heavy Chain), under reducing conditions; 199.5 kDa, under non-reducing conditions. |
| Purification Method | Protein A purified |
| Purity | >95% |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle. |
| CAS | 1430205-07-4 |
Imalumab (anti-MIF) (Ab177902) - ELISA
Immobilized Recombinant Human MIF protein (rp148808) at 1.0 μg/mL can bind Imalumab (anti-MIF) (Ab177902) with the EC50 of 108.7 ng/mL.
Imalumab (anti-MIF) (Ab177902) - SEC
The purity of Imalumab (anti-MIF) (Ab177902) is more than 95% verified by HPLC.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0707793 | Certificate of Analysis | Jul 04, 2024 | Ab177902 |
| ZJ24F0707792 | Certificate of Analysis | Jul 04, 2024 | Ab177902 |
| ZJ24F0707791 | Certificate of Analysis | Jul 04, 2024 | Ab177902 |
| 1. Leng L, Metz CN, Fang Y, Xu J, Donnelly S, Baugh J, Delohery T, Chen Y, Mitchell RA, Bucala R. (2003) MIF signal transduction initiated by binding to CD74.. J Exp Med, 197 (11): (1467-76). [PMID:12782713] |
| 2. Trivedi-Parmar V, Jorgensen WL. (2018) Advances and Insights for Small Molecule Inhibition of Macrophage Migration Inhibitory Factor.. J Med Chem, 61 (18): (8104-8119). [PMID:29812929] |
| 3. Lubetsky JB, Swope M, Dealwis C, Blake P, Lolis E. (1999) Pro-1 of macrophage migration inhibitory factor functions as a catalytic base in the phenylpyruvate tautomerase activity.. Biochemistry, 38 (22): (7346-54). [PMID:10353846] |
| 4. Orita M, Yamamoto S, Katayama N, Aoki M, Takayama K, Yamagiwa Y, Seki N, Suzuki H, Kurihara H, Sakashita H et al.. (2001) Coumarin and chromen-4-one analogues as tautomerase inhibitors of macrophage migration inhibitory factor: discovery and X-ray crystallography.. J Med Chem, 44 (4): (540-7). [PMID:11170644] |
| 5. Ouertatani-Sakouhi H, El-Turk F, Fauvet B, Cho MK, Pinar Karpinar D, Le Roy D, Dewor M, Roger T, Bernhagen J, Calandra T, Zweckstetter M, Lashuel HA. () Identification and characterization of novel classes of macrophage migration inhibitory factor (MIF) inhibitors with distinct mechanisms of action.. J Biol Chem, [PMID:20516071] |
| 6. Gregg V Crichlow,Jodi B Lubetsky,Lin Leng,Richard Bucala,Elias J Lolis. (2008-12-19) Structural and kinetic analyses of macrophage migration inhibitory factor active site interactions.. Biochemistry, 48 ((1)): (132-139). [PMID:19090677] |
| 7. Alissa A Hare,Lin Leng,Sunilkumar Gandavadi,Xin Du,Zoe Cournia,Richard Bucala,William L Jorgensen. (2010-08-24) Optimization of N-benzyl-benzoxazol-2-ones as receptor antagonists of macrophage migration inhibitory factor (MIF).. Bioorganic & medicinal chemistry letters, 20 ((19)): (5811-5814). [PMID:20728358] |
| 8. William L Jorgensen,Alexander Trofimov,Xin Du,Alissa A Hare,Lin Leng,Richard Bucala. (2011-07-02) Benzisothiazolones as modulators of macrophage migration inhibitory factor.. Bioorganic & medicinal chemistry letters, 21 ((15)): (4545-4549). [PMID:21719283] |
| 9. Crichlow GV, Cheng KF, Dabideen D, Ochani M, Aljabari B, Pavlov VA, Miller EJ, Lolis E, Al-Abed Y.. (2007) Alternative chemical modifications reverse the binding orientation of a pharmacophore scaffold in the active site of macrophage migration inhibitory factor.. J Biol Chem, 282 (32): (23089-23095). [PMID:17526494] |
| 10. Cournia Z, Leng L, Gandavadi S, Du X, Bucala R, Jorgensen WL.. (2009) Discovery of human macrophage migration inhibitory factor (MIF)-CD74 antagonists via virtual screening.. J Med Chem, 52 (2): (416-424). [PMID:19090668] |
| 11. El Turk F, Fauvet B, Ouertatani-Sakouhi H, Lugari A, Betzi S, Roche P, Morelli X, Lashuel HA.. (2010) An integrative in silico methodology for the identification of modulators of macrophage migration inhibitory factor (MIF) tautomerase activity.. Bioorg Med Chem, 18 (14): (5425-5440). [PMID:20639113] |
| 12. Jorgensen WL, Gandavadi S, Du X, Hare AA, Trofimov A, Leng L, Bucala R.. (2010) Receptor agonists of macrophage migration inhibitory factor.. Bioorg Med Chem Lett, 20 (23): (7033-7036). [PMID:20971005] |
| 13. Alam A, Pal C, Goyal M, Kundu MK, Kumar R, Iqbal MS, Dey S, Bindu S, Sarkar S, Pal U, Maiti NC, Adhikari S, Bandyopadhyay U.. (2011) Synthesis and bio-evaluation of human macrophage migration inhibitory factor inhibitor to develop anti-inflammatory agent.. Bioorg Med Chem, 19 (24): (7365-7373). [PMID:22088307] |
| 14. Dahlgren MK, Garcia AB, Hare AA, Tirado-Rives J, Leng L, Bucala R, Jorgensen WL.. (2012) Virtual screening and optimization yield low-nanomolar inhibitors of the tautomerase activity of Plasmodium falciparum macrophage migration inhibitory factor.. J Med Chem, 55 (22): (10148-10159). [PMID:23067344] |
| 15. Yu WL, Jones BD, Kang M, Hammons JC, La Clair JJ, Burkart MD.. (2013) Spirohexenolide A targets human macrophage migration inhibitory factor (hMIF).. J Nat Prod, 76 (5): (817-823). [PMID:23659282] |
| 16. Pantouris G, Rajasekaran D, Garcia AB, Ruiz VG, Leng L, Jorgensen WL, Bucala R, Lolis EJ.. (2014) Crystallographic and receptor binding characterization of Plasmodium falciparum macrophage migration inhibitory factor complexed to two potent inhibitors.. J Med Chem, 57 (20): (8652-8656). [PMID:25268646] |
| 17. Spencer ES, Dale EJ, Gommans AL, Rutledge MT, Vo CT, Nakatani Y, Gamble AB, Smith RA, Wilbanks SM, Hampton MB, Tyndall JD.. (2015) Multiple binding modes of isothiocyanates that inhibit macrophage migration inhibitory factor.. Eur J Med Chem, 93 (501-510). [PMID:25743213] |
| 18. Le Hiress M, Akagah B, Bernadat G, Tu L, Thuillet R, Huertas A, Phan C, Fadel E, Simonneau G, Humbert M, Jalce G, Guignabert C.. (2018) Design, Synthesis, and Biological Activity of New N-(Phenylmethyl)-benzoxazol-2-thiones as Macrophage Migration Inhibitory Factor (MIF) Antagonists: Efficacies in Experimental Pulmonary Hypertension.. J Med Chem, 61 (7): (2725-2736). [PMID:29526099] |
| 19. Kok T, Wapenaar H, Wang K, Neochoritis CG, Zarganes-Tzitzikas T, Proietti G, Eleftheriadis N, Kurpiewska K, Kalinowska-Tłuścik J, Cool RH, Poelarends GJ, Dömling A, Dekker FJ.. (2018) Discovery of chromenes as inhibitors of macrophage migration inhibitory factor.. Bioorg Med Chem, 26 (5): (999-1005). [PMID:29428527] |
| 20. Cirillo PF,Asojo OA,Khire U,Lee Y,Mootien S,Hegan P,Sutherland AG,Peterson-Roth E,Ledizet M,Koski RA,Anthony KG. (2020) Inhibition of Macrophage Migration Inhibitory Factor by a Chimera of Two Allosteric Binders.. ACS Med Chem Lett, 11 (10.0): (1843-1847). [PMID:33062162] |
| 21. Xiao Z,Fokkens M,Chen D,Kok T,Proietti G,van Merkerk R,Poelarends GJ,Dekker FJ. (2020) Structure-activity relationships for binding of 4-substituted triazole-phenols to macrophage migration inhibitory factor (MIF).. Eur J Med Chem, 186 (111849-111849). [PMID:31767137] |
| 22. Hochstrasser, D F DF and 9 more authors.. (1992) Human liver protein map: a reference database established by microsequencing and gel comparison.. Electrophoresis, [PMID:1286669] |
| 23. Weiser, W Y WY and 5 more authors.. (1989) Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2552447] |
| 24. Wistow, G J GJ, Shaughnessy, M P MP, Lee, D C DC, Hodin, J J and Zelenka, P S PS.. (1993) A macrophage migration inhibitory factor is expressed in the differentiating cells of the eye lens.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:7679497] |
| 25. Zeng, F Y FY and 5 more authors.. (1993) The major binding protein of the interferon antagonist sarcolectin in human placenta is a macrophage migration inhibitory factor.. Archives of biochemistry and biophysics, (15): [PMID:7683862] |
| 26. Bernhagen, J J and 5 more authors.. (1994) Purification, bioactivity, and secondary structure analysis of mouse and human macrophage migration inhibitory factor (MIF).. Biochemistry, (29): [PMID:7947826] |
| 27. Paralkar, V V and Wistow, G G.. (1994) Cloning the human gene for macrophage migration inhibitory factor (MIF).. Genomics, (1): [PMID:8188240] |
| 28. Mikayama, T T and 9 more authors.. (1993) Molecular cloning and functional expression of a cDNA encoding glycosylation-inhibiting factor.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:8234256] |
| 29. Kato, Y Y and 7 more authors.. (1996) The crystal structure of human glycosylation-inhibiting factor is a trimeric barrel with three 6-stranded beta-sheets.. Proceedings of the National Academy of Sciences of the United States of America, (2): [PMID:8610159] |
| 30. Sun, H W HW, Bernhagen, J J, Bucala, R R and Lolis, E E.. (1996) Crystal structure at 2.6-A resolution of human macrophage migration inhibitory factor.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:8643551] |
| 31. Sugimoto, H H, Suzuki, M M, Nakagawa, A A, Tanaka, I I and Nishihira, J J.. (1996) Crystal structure of macrophage migration inhibitory factor from human lymphocyte at 2.1 A resolution.. FEBS letters, (1): [PMID:8766818] |
| 32. Kleemann, R R and 13 more authors.. (2000) Intracellular action of the cytokine MIF to modulate AP-1 activity and the cell cycle through Jab1.. Nature, (9): [PMID:11089976] |
| 33. . (2001) A novel 5'-flanking region polymorphism of macrophage migration inhibitory factor is associated with systemic-onset juvenile idiopathic arthritis.. Arthritis and rheumatism, [PMID:11508429] |
| 34. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801] |
| 35. Shen, Li L and 7 more authors.. (2003) The apoptosis-associated protein BNIPL interacts with two cell proliferation-related proteins, MIF and GFER.. FEBS letters, (10): [PMID:12681488] |
| 36. Emonts, Marieke M and 10 more authors.. (2007) Association between high levels of blood macrophage migration inhibitory factor, inappropriate adrenal response, and early death in patients with severe sepsis.. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, (15): [PMID:17443469] |
| 37. Merk, Melanie M and 13 more authors.. (2009) The Golgi-associated protein p115 mediates the secretion of macrophage migration inhibitory factor.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:19454686] |
| 38. Alam, Athar A and 12 more authors.. (2012) Novel anti-inflammatory activity of epoxyazadiradione against macrophage migration inhibitory factor: inhibition of tautomerase and proinflammatory activities of macrophage migration inhibitory factor.. The Journal of biological chemistry, (13): [PMID:22645149] |
| 39. Fan, Chengpeng C and 7 more authors.. (2013) MIF intersubunit disulfide mutant antagonist supports activation of CD74 by endogenous MIF trimer at physiologic concentrations.. Proceedings of the National Academy of Sciences of the United States of America, (2): [PMID:23776208] |
