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Isopropyl β-D-thiogalactopyranoside solution - ReadyMade IPTG solution for Blue-white screening, high purity , CAS No.367-93-1

  • ReadyMade IPTG solution for Blue-white screening
Item Number
I485502
Grouped product items
SKUSizeAvailabilityPrice Qty
I485502-5ml
5ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$191.90

Basic Description

SynonymsIPTG|367-93-1|Isopropyl-beta-D-thiogalactopyranoside|Isopropyl beta-D-thiogalactopyranoside|ISOPROPYL THIOGALACTOSIDE|Isopropyl-beta-D-thiogalactoside|Isopropyl beta-D-1-thiogalactopyranoside|Isopropyl beta-D-thiogalactoside|(2R,3R,4S,5R,6S)-2-(Hydroxymet
Specifications & PurityReadyMade IPTG solution for Blue-white screening
Storage TempStore at -20°C
Shipped InDry ice
Product Description

Description

Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins inE. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter. IPTG Ready Made is a 0.2 μm filter sterilized solution of 200 mM IPTG. This reagent remains liquid at its storage temperature (-20 °C), thus avoiding freeze-thaw cycles and providing a user-friendly solution.Component:IPTG Solution is composed of 200 mM IPTG in molecular biology grade water.Isopropyl β-D-thiogalactopyranoside solution is:used as an inducer for the expression of single domain antibodies(sdAbs) inE coliand bioactive peptidesused in its half-life and decay studiessuitable for induction of recombinant protein expression in systems controlled by the lac repressor


Preparation instructions



Principle

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β-gal

Names and Identifiers

IUPAC Name (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-propan-2-ylsulfanyloxane-3,4,5-triol
INCHI InChI=1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
InChi Key BPHPUYQFMNQIOC-NXRLNHOXSA-N
Canonical SMILES CC(C)SC1C(C(C(C(O1)CO)O)O)O
Isomeric SMILES CC(C)S[C@H]1[C@@H]([C@H]([C@H]([C@H](O1)CO)O)O)O
WGK Germany 2
PubChem CID 656894
Molecular Weight 238.3
Beilstein 4631
Reaxy-Rn 4631

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Chemical and Physical Properties

Specific Rotation[α]-32° (C=1,H2O)
Melt Point(°C)109-121℃

Safety and Hazards(GHS)

Pictogram(s) GHS08,   GHS07
Signal Warning
Hazard Statements

H315:Causes skin irritation

H319:Causes serious eye irritation

H335:May cause respiratory irritation

H351:Suspected of causing cancer

H302:Harmful if swallowed

H312:Harmful in contact with skin

H332:Harmful if inhaled

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

P280:Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352:IF ON SKIN: wash with plenty of water.

P321:Specific treatment (see ... on this label).

P405:Store locked up.

P501:Dispose of contents/container to ...

P264:Wash hands [and …] thoroughly after handling.

P271:Use only outdoors or in a well-ventilated area.

P270:Do not eat, drink or smoke when using this product.

P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing.

P403+P233:Store in a well-ventilated place. Keep container tightly closed.

P362+P364:Take off contaminated clothing and wash it before reuse.

P330:Rinse mouth.

P203:Obtain, read and follow all safety instructions before use.

P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes.

P301+P317:IF SWALLOWED: Get medical help.

P318:if exposed or concerned, get medical advice.

P317:Get emergency medical help.

P337+P317:If eye irritation persists: Get medical help.

P332+P317:If skin irritation occurs: Get medical help.

P319:Get medical help if you feel unwell.

WGK Germany 2
Reaxy-Rn 4631
Merck Index 5082

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References

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