The Jurkat whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis, and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.
Specifications & Purity
Carrier Free, Azide Free, 200μg, Mycoplasma free
Images
Jurkat Whole Cell Lysate (cl155897) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: Jurkat Whole Cell Lysate (cl155897) Lane 2: MOLT-4 Whole Cell Lysate (cl155905) Lane 3: LNCaP Whole Cell Lysate (cl155902) Lane 4: Ramos Whole Cell Lysate (cl155908)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa Observed band size: 42 kDa
Jurkat Whole Cell Lysate (cl155897) - Western Blot All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: Jurkat Whole Cell Lysate (cl155897) Lane 2: MOLT-4 Whole Cell Lysate (cl155905) Lane 3: LNCaP Whole Cell Lysate (cl155902) Lane 4: Ramos Whole Cell Lysate (cl155908)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa Observed band size: 29 kDa
Jurkat Whole Cell Lysate (cl155897) - Western Blot All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: Jurkat Whole Cell Lysate (cl155897) Lane 2: MOLT-4 Whole Cell Lysate (cl155905) Lane 3: LNCaP Whole Cell Lysate (cl155902) Lane 4: Ramos Whole Cell Lysate (cl155908)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa Observed band size: 58 kDa
Product Specifications
Form
Lyophilized
Concentration
200μg, Mycoplasma free
Reconstitution
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white pow
Storage Temp
Store at -80°C
Shipped In
Ice chest + Ice pads
Stability And Storage
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
Certificates
Certificate of Analysis(COA)
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ZJ23F0700548