The K-562 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
K-562 is an erythroleukemia cell line derived from a chronic myeloid leukemia patient in blast crisis. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. Cells are also used for haemoglobin synthesis, antitumour testing, tumorigenicity, differentiation, pharmacodynamics, cloning, cytotoxicity, cell biology, cellular effects of hyperthermia, and natural killer assays.
Specifications & Purity
Carrier Free, Azide Free, 200μg, Mycoplasma free
Images
K562 Whole Cell Lysate (cl155898) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: K562 Whole Cell Lysate (cl155898) Lane 2: HEK293 Whole Cell Lysate (cl155895) Lane 3: A431 Whole Cell Lysate (cl155901) Lane 4: NIH/3T3 Whole Cell Lysate (cl155900) Lane 5: HT-29 Whole Cell Lysate (cl155904)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa Observed band size: 42 kDa
K-562 Whole Cell Lysate (cl155898) - Western Blot All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: K-562 Whole Cell Lysate (cl155898) Lane 2: HEK-293 Whole Cell Lysate (cl155895) Lane 3: A431 Whole Cell Lysate (cl155901) Lane 4: NIH/3T3 Whole Cell Lysate (cl155900) Lane 5: HT-29 Whole Cell Lysate (cl155904)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa Observed band size: 29 kDa
K-562 Whole Cell Lysate (cl155898) - Western Blot All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: K-562 Whole Cell Lysate (cl155898) Lane 2: HEK-293 Whole Cell Lysate (cl155895) Lane 3: A431 Whole Cell Lysate (cl155901) Lane 4: NIH/3T3 Whole Cell Lysate (cl155900) Lane 5: HT-29 Whole Cell Lysate (cl155904)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa Observed band size: 58 kDa
Product Specifications
Form
Lyophilized
Concentration
200μg, Mycoplasma free
Reconstitution
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white pow
Storage Temp
Store at -80°C
Shipped In
Ice chest + Ice pads
Stability And Storage
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
Certificates
Certificate of Analysis(COA)
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ZJ23F0800805