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Lipopolysaccharide, E. coli 055:B5 - purified by trichloroacetic acid extraction, high purity , CAS No.93572-42-0

74 Citations
  • purified by trichloroacetic acid extraction
Item Number
L386714
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L386714-5mg
5mg
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$85.90
L386714-10mg
10mg
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$136.90
L386714-25mg
25mg
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a component of the cell wall of Gram-negative bacteria and Specific activator of Toll-like receptor (TLR) 4.

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Polysaccharide

Basic Description

SynonymsLPS
Specifications & Puritypurified by trichloroacetic acid extraction
Biochemical and Physiological MechanismsPhysiol Actions：Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of b
Storage TempStore at 2-8°C
Shipped InWet ice
Product Description

Lipopolysaccharide,|E. coli|O55:B5 is a component of the cell wall of the O55:B5 serotype of the Gram-negative bacteria,|Escherichia coli|. Lipopolysaccharides (LPS) are made up of a hydrophobic lipid, a hydrophilic core polysaccharide chain and a hydrophilic O-antigenic polysaccharide side chain. LPS molecules contribute to the outer integrity of the cell membrane, protect the cell against bile salts and lipophilic antibiotics, and act as endotoxins in animals. LPS induces a strong immune response by binding to the CD14/TLR4/MD2 receptor complex, which promotes the secretion of pro-inflammatory cytokines. LPS is also an exogenous pyrogen. LPS O55:B5 has been derived from a serotype of|E. coli|O55:B5, which is part of a subdivision based on the O antigen component of LPS. Lipopolysaccharide, E. coli O55:B5 is a form of lipopolysaccharide (LPS) extracted from wild-type S-form E. coli serotype O55:B5. Lipopolysaccharide, E. coli O55:B5 is commonly used to activate toll-like receptor 4 (TLR4) on leukocytes, eliciting inflammatory signaling in isolated cells and in vivo.1,2,3,4 Lipopolysaccharide, E. coli O55:B5 includes three regions, the O-antigenic polysaccharide chain, the core oligosaccharide, and lipid A. Lipopolysaccharide, E. coli O55:B5 stimulates the activity of inducible nitric oxide synthase. Lipopolysaccharide, E. coli O55:B5 induces apoptosis in mouse thymus and swine lymphocytes. Lipopolysaccharide, E. coli O55:B5 is also known as LPS. Lipopolysaccharide, E. coli O55:B5is a component of the cell wall of Gram-negative bacteria and Specific activator of Toll-like receptor (TLR) 4.

Usage:

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Certificates

Certificate of Analysis(COA)

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19 results found

Lot NumberCertificate TypeDateItem
E2417232Certificate of AnalysisApr 18, 2024 L386714
E2417252Certificate of AnalysisApr 18, 2024 L386714
D2407378Certificate of AnalysisMar 09, 2024 L386714
L2410279Certificate of AnalysisDec 01, 2023 L386714
C2415110Certificate of AnalysisDec 01, 2023 L386714
L2315522Certificate of AnalysisDec 01, 2023 L386714
L2315521Certificate of AnalysisDec 01, 2023 L386714
L2315520Certificate of AnalysisDec 01, 2023 L386714
E2326858Certificate of AnalysisMay 17, 2023 L386714
E2326857Certificate of AnalysisMay 17, 2023 L386714
E2326856Certificate of AnalysisMay 17, 2023 L386714
E2326852Certificate of AnalysisMay 17, 2023 L386714
F23081009Certificate of AnalysisSep 27, 2022 L386714
F23081014Certificate of AnalysisSep 27, 2022 L386714
L2202167Certificate of AnalysisSep 27, 2022 L386714
L2202179Certificate of AnalysisSep 27, 2022 L386714
H2223374Certificate of AnalysisJul 30, 2022 L386714
H2223446Certificate of AnalysisJul 30, 2022 L386714
H2223447Certificate of AnalysisJul 30, 2022 L386714

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Chemical and Physical Properties

SolubilitySoluble in water (5 mg/ml).

Related Documents

 SDS

 Specification Sheet

FAQs and Articles

FAQ
Lipopolysaccharide (LPS): The Bacterial Outer Membrane
Categories: Technical articles
Tags:
Natural products Carbohydrates Lipids Bioactive Small Molecules Lipopolysaccharide Bacteria Immune recognition Microbiology Immunology

Citations of This Product

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