Lipopolysaccharides from Escherichia coli O55:B5

Item Number

L755740

• Specifications & Purity: γ-irradiated, BioReagent, for cell culture
• Biochemical and Physiological Mechanisms: LPS (lipopolysaccharide) is a major constituent of the outer membrane of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. The LPS confers antigenic and endotoxic characters t
• Storage Temp: Store at 2-8°C
• Shipped In: Wet ice
• Grade: BioReagent, for cell culture, γ-irradiated

Product Description

Lipopolysaccharides (LPS) are complex glycolipids that contain several components. Enterobacteriaceae, such as E. coli, have highly conserved lipid A linked to the antigenic O-polysaccharide. Hydrophobic lipid A forms the outer most component that plays a role in the biological properties attributed to endotoxic LPS. A short core oligosaccharide (core OS) is found to be attached to lipid A. Enterobacteria contain an antigenic, repeat-structure O-polysaccharide (O-PS) attached to the distal core OS to form smooth LPS. O-PS in E. coli contains ∼170 distinct antigens that interact with the host immune system. Based on the core OS structures in E. coli, they have been designated K-12 and R1 to R4.

Application:

LPS (lipopolysaccharide) from Escherichia coli 055:B5 has been used:

• to stimulate human PBMC to secrete cytokine.
• to stimulate wild type embryonic fibroblasts and mutants for the induction of phosphorylation of p56
• in the measurement of tumor necrosis factor-α levels and nuclear factor-κB p65 activities after stimulation
• in the stimulation of hamster lymphocytes in vitro
• to induce activated dendritic morphology in murine macrophage cell line in a study

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).