Lyso-Tracker Green

Item Number

L747762

• Stability And Storage: Store at -20℃ in the dark for up to 6 months.
• Storage Temp: Protected from light,Store at -20°C
• Shipped In: Ice chest + Ice pads

Product Description

Precautions：

Lyso-Tracker Green (1mM) will solidify on ice or at lower temperatures such as 4℃ and stick to the bottom, walls or lid of the centrifuge tube. It should be thawed completely in a water bath at 20-25℃ prior to use. For trace amounts of liquid, centrifuge briefly before each use to collect the liquid at the bottom of the tube.Fluorescence intensity decays. Avoid light to minimize the quenching of fluorescence. 3% glutaraldehyde can be attempted to fix Lyso-Tracker Green-stained cells.Coverslips and slides are required but not supplied. They can be ordered from .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1.Preparation of Lyso-Tracker Green working solutiona.Dilute an appropriate amount of Lyso-Tracker Green (1mM) in cell culture medium or other appropriate solutions (e.g., HBSS with Ca2+ & Mg2+) at a ratio of 1:13333-1:20000 to a final concentration of 50-75nM. For example, mix 1µl of Lyso-Tracker Green (1mM) with 20ml or 13.33ml of dilution buffer to obtain the Lyso-Tracker Green working solution. HBSS with Ca2+ & Mg2+ can be ordered from .b.Prewarm the Lyso-Tracker Green working solution at 37℃ before use.Note: The concentration of Lyso-Tracker Green in the working solution can be adjusted appropriately according to the staining results.To minimize the staining background, a lower concentration of Lyso-Tracker Green is preferred as long as an acceptable staining result can be obtained.2.Fluorescent labelling of lysosomea.Remove the cell culture medium, add Lyso-Tracker Green working solution pre-incubated at 37℃, and incubate at 37℃ for 5-60 min.b.Remove the Lyso-Tracker Green working solution and add fresh cell culture medium.c.Examine cells by fluorescence microscope or confocal microscope. If the staining is too weak, increase the concentration of Lyso-Tracker Green or extend the staining time appropriately within the recommended time period.Related Products：Cat. No.Product NamePack Size C1002 DAPI 5mg/ml×0.2ml C1005/C1006 DAPI staining solution 10ml/50ml C1011 Hoechst 33258 10mg C1017/C1018 Hoechst 33258 Staining Solution 10ml/50ml C1022 Hoechst 33342 10mg C1025/C1026 Hoechst 33258 Staining Solution 10ml/50ml C1027/C1028/C1029 Hoechst 33342 Staining Solution for Live Cells (100X) 0.1ml/0.5ml/3ml C1033 Actin-Tracker Green (microfilament green fluorescent probe) 0.2ml C1036 DiI (cell membrane red fluorescent probe) 10mg C1038 DiO (cell membrane green fluorescent probe) 10mg C1039-10mg DiD (cell membrane far-infrared fluorescent probe) 10mg C1041 ER-Tracker Red (Endoplasmic Reticulum Red Fluorescent Probe) 20μl C1042S ER-Tracker Green (Endoplasmic Reticulum Green Fluorescent Probe) 20μl C1043 Golgi-Tracker Red (Golgi red fluorescent probe) 1mg C1045S Golgi-Tracker Green (Golgi green fluorescent probe) 1mg C1046 Lyso-Tracker Red (Lysosomal Red Fluorescent Probe) 50μl C1047S Lyso-Tracker Green (lysosomal green fluorescent probe) 50μl C1048 Mito-Tracker Green (mitochondrial green fluorescent probe) 50μg C1049-50µg Mito-Tracker Red CMXRos (Mitochondrial Red Fluorescent Probes) 50μg C1049-250µg Mito-Tracker Red CMXRos (Mitochondrial Red Fluorescent Probes) 50μg×5 C1050 Tubulin-Tracker Red (antibody-based microtubule red fluorescent probe) 40μl C1051S Tubulin-Tracker Green (antibody-based microtubule green fluorescent probe) 40μl C1991S Red fluorescence staining kit for cell membrane (DiI) 200-1000T C1993S Green fluorescent staining kit for cell membranes (DiO) 200-1000T C1995S Far-infrared fluorescence staining kit for cell membrane (DiD) 200-1000T C2005 JC-1 1mg C2007 Rhodamine 123 5mg