MCF7 Whole Cell Lysateused for WB various applications
Grade
Azide Free, Carrier Free
Product Description
Product Description:
The MCF7 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
MCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.
Specifications & Purity
Carrier Free, Azide Free, 200μg, Mycoplasma free
Product Specifications
Application
WB
Form
Lyophilized
Concentration
200μg, Mycoplasma free
Reconstitution
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white po
Storage Temp
Store at -80°C
Shipped In
Ice chest + Ice pads
Certificates
Certificate of Analysis(COA)
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