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Methyl Blue - Biological Stain, high purity , CAS No.28983-56-4

  • Biological stain
Item Number
M278712
Grouped product items
SKUSizeAvailabilityPrice Qty
M278712-25g
25g
In stock
$52.90
M278712-100g
100g
In stock
$119.90
M278712-500g
500g
Available within 8-12 weeks(?)
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$399.90

Histology stain for collagen.

View related series
Organic dyes

Basic Description

Synonyms28983-56-4|Acid Blue 93|C.I. Acid Blue 93|water blue for microscopy|MFCD00058509|6415-98-1|Benzenesulfonic acid,[[4-[bis[4-[(sulfophenyl)amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]amino]-,sodium salt (1:2)|disodium;2-[4-[[4-(2-sulfoanilino)phenyl
Specifications & PurityBiological stain
Biochemical and Physiological MechanismsMethyl Blue is considered as a xenobiotic compound and has antiseptic property. It is useful in polychrome staining. Methyl blue is mostly used for staining samples in histology and microbiology. It is also useful as a model to reveal the role of catalyst
Storage TempDesiccated
Shipped InNormal
GradeBiological stain
NoteWherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description

Methyl Blue is a histology dye commonly used to stain collagen blue in tissue sections. The compound has been determined useful as a vital dye for interocular observations. Methyl Blue has been incorporated in Sabouraud agar for the identification of Candida albicans strains. The compound has also been incorporated in other histology staining procedures such as Mallory’s connective tissue stain.
A histology stain for collagen in tissue sections

Methyl Blue has been used:

· to stain leaf disks for callose visualization within the cells

· to incubate cells for 4′,6-diamidino-2-phenylindole (DAPI)/septa staining

· as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin


application:

hematology: suitable;histology: suitable

Names and Identifiers

Pubchem Sid488198683
Pubchem Sid Urlhttps://pubchem.ncbi.nlm.nih.gov/substance/488198683
IUPAC Name disodium;4-[4-[[4-(4-sulfoanilino)phenyl]-[4-(4-sulfonatophenyl)iminocyclohexa-2,5-dien-1-ylidene]methyl]anilino]benzenesulfonate
INCHI InChI=1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2
InChi Key MCPLVIGCWWTHFH-UHFFFAOYSA-L
Canonical SMILES C1=CC(=NC2=CC=C(C=C2)S(=O)(=O)[O-])C=CC1=C(C3=CC=C(C=C3)NC4=CC=C(C=C4)S(=O)(=O)O)C5=CC=C(C=C5)NC6=CC=C(C=C6)S(=O)(=O)[O-].[Na+].[Na+]
Isomeric SMILES C1=CC(=NC2=CC=C(C=C2)S(=O)(=O)[O-])C=CC1=C(C3=CC=C(C=C3)NC4=CC=C(C=C4)S(=O)(=O)O)C5=CC=C(C=C5)NC6=CC=C(C=C6)S(=O)(=O)[O-].[Na+].[Na+]
WGK Germany 3
PubChem CID 14513727
Molecular Weight 799.8

Certificates

Certificate of Analysis(COA)

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14 results found

Lot NumberCertificate TypeDateItem
I2306556Certificate of AnalysisAug 24, 2023 M278712
I2306662Certificate of AnalysisAug 24, 2023 M278712
B2314138Certificate of AnalysisJul 23, 2022 M278712
D2307457Certificate of AnalysisJul 23, 2022 M278712
H2311016Certificate of AnalysisJul 23, 2022 M278712
H2311019Certificate of AnalysisJul 23, 2022 M278712
I2207910Certificate of AnalysisJul 23, 2022 M278712
I2207936Certificate of AnalysisJul 23, 2022 M278712
I2207937Certificate of AnalysisJul 23, 2022 M278712
B2218401Certificate of AnalysisJan 25, 2022 M278712
B2218452Certificate of AnalysisJan 25, 2022 M278712
B2218533Certificate of AnalysisJan 25, 2022 M278712
B2314139Certificate of AnalysisJan 25, 2022 M278712
G2226077Certificate of AnalysisJan 25, 2022 M278712

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Chemical and Physical Properties

Solubilitywater: 0.01 g/10 mL, blue to very deep blue

Safety and Hazards(GHS)

Pictogram(s) GHS07
Hazard Statements

H315:Causes skin irritation

H319:Causes serious eye irritation

H335:May cause respiratory irritation

H302:Harmful if swallowed

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

WGK Germany 3

Related Documents

Solution Calculators