28983-56-4|Acid Blue 93|C.I. Acid Blue 93|water blue for microscopy|MFCD00058509|6415-98-1|Benzenesulfonic acid,[[4-[bis[4-[(sulfophenyl)amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]amino]-,sodium salt (1:2)|disodium;2-[4-[[4-(2-sulfoanilino)phenyl
Specifications & Purity
Biological stain
Biochemical and Physiological Mechanisms
Methyl Blue is considered as a xenobiotic compound and has antiseptic property. It is useful in polychrome staining. Methyl blue is mostly used for staining samples in histology and microbiology. It is also useful as a model to reveal the role of catalyst
Storage Temp
Desiccated
Shipped In
Normal
Grade
Biological stain
Note
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description
Methyl Blue is a histology dye commonly used to stain collagen blue in tissue sections. The compound has been determined useful as a vital dye for interocular observations. Methyl Blue has been incorporated in Sabouraud agar for the identification of Candida albicans strains. The compound has also been incorporated in other histology staining procedures such as Mallory’s connective tissue stain. A histology stain for collagen in tissue sections
Methyl Blue has been used:
· to stain leaf disks for callose visualization within the cells
· to incubate cells for 4′,6-diamidino-2-phenylindole (DAPI)/septa staining
· as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin