ExactAb™, Validated, Carrier Free, Azide Free, ≥95%(SDS-PAGE), Lot by Lot
Host species
Rat
Specificity
MHC Class I/H-2
Immunogen
C57BL/10 mouse spleen cells enriched for T cells
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, Validated
Product Description
Rat anti Mouse MHC Class I/H-2 Antibody, Monoclonal (M1/42.3.9.8), could be used for ex vivo blocking of MHC I-dependent interactions, ICC, IF, Flow and so on. Application: ex vivo blocking of MHC I-dependent interactions: Use at an assay dependent concentration. ICC/IF: Use at an assay dependent concentration. Flow: Use at an assay dependent concentration.
Product Properties
Isotype
Rat IgG2a
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at 2-8°C,Freeze prohibited
Shipped In
Wet ice
Stability And Storage
Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon delivery aliquot.
Images
MHC Class I/H-2 Rat mAb (Ab108165) - Flow Cytometry Flow Cytometry analysis of mouse splenocytes cells labelling MHC Class I/H-2 with MHC Class I/H-2 Rat mAb (Ab108165) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).
MHC Class I/H-2 Rat mAb (Ab108165) - Flow Cytometry Flow Cytometry analysis of mouse splenocytes cells labelling MHC Class I/H-2 (red) with MHC Class I/H-2 Rat mAb (Ab108165) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
ICC/IF
Use at an assay dependent concentration.
Flow
Use at an assay dependent concentration.
ex vivo blocking of MHC I-dependent interactions
Use at an assay dependent concentration.
Certificates
Certificate of Analysis(COA)
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