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MHC Class II Rat mAb - Primary antibody, specific to MHC Class II, Rat IgG2b

Azide Free
Carrier Free
ExactAb™
Validated
Lot by Lot

Features and benefits

Short Overview:
mAb (M5/114.15.2); Rat anti Mouse MHC Class II Antibody; Flow; Unconjugated
Species reactivity(Reacts with): Mouse
Isotype: Rat IgG2b

Application:

Flow Cytometry

Item Number

Ab115282

Grouped product items
SKU	Size	Availability	Price
Ab115282-10μg	10μg	In stock	$19.90
Ab115282-50μg	50μg	In stock	$59.90
Ab115282-100μg	100μg	In stock	$109.90
Ab115282-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$499.90

mAb (M5/114.15.2); Rat anti Mouse MHC Class II Antibody; Flow; Unconjugated

View related series

Accession#:O78196 Accession#:P14483 Gene ID:14961 Gene ID:14969 H2-Ab1 H2-Eb1 MHC Class II

Basic Description

Product Name	MHC Class II Rat mAb - Primary antibody, specific to MHC Class II, Rat IgG2b
Synonyms	E-alpha-f;H-2 class II histocompatibility antigen, E-K alpha chain;H-2 class II histocompatibility antigen, E-U alpha chain;H-2E;H2-Ea;H-2Ea;H2-Ea-ps;I;Ia;Ia3;Ia-3;I-E;I-Ealpha;MHC-H2-Ea; H2-Ea; MHC class II (I-A/I-E); MHC class II IA/IE; D6S221E antibody
Specifications & Purity	ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host species	Rat
Specificity	MHC Class II
Immunogen	Activated C57BL/6 mouse spleen cells
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, Validated
Product Description	Rat anti Mouse MHC Class II Antibody, Monoclonal (M5/114.15.2), could be used for Flow and so on. Application: Flow: 1.0 μg/100w cells Protein Function Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Isotype

Rat IgG2b

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Form

Liquid

Concentration

Lot by Lot

Storage Temp

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot.

Images

MHC Class II Rat mAb (Ab115282) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling MHC Class II with MHC Class II Rat mAb (Ab115282) conjugated with biotin (right panel) at 0.02 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - Isotype Control (left panel) and detected with SA-PE (rp156250).

MHC Class II Rat mAb (Ab115282) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling MHC Class II (red) with MHC Class II Rat mAb (Ab115282) conjugated with biotin at 0.02 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Dilution info

Flow

1μg/100w cells

Certificates

Certificate of Analysis(COA)

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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0505803	Certificate of Analysis	May 20, 2024	Ab115282
ZJ24F0505804	Certificate of Analysis	May 20, 2024	Ab115282
ZJ24F0505805	Certificate of Analysis	May 20, 2024	Ab115282

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