Mouse Anti-Human IgG Antibody - Secondary antibody, for ELISA,CLIA various applications, from Human, generated in Rat, specific to Human IgG, IgG1
Synonyms
Mouse Anti-Human IgG Antibody | Mouse Anti Human IgG Antibody | Anti-Human IgG Antibody | Anti Human IgG Antibody | Human IgG Antibody | FLJ39988 antibodyFLJ40036 antibody | FLJ40253 antibody | FLJ40587 antibody | FLJ40789 antibody | FLJ40834 antibody | G
Specifications & Purity
ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host Species
Rat
Host species
Mouse
Application
ELISA,CLIA
Immunogen
Human IgG
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, Validated
Product Description
Mouse Anti-Human IgG Antibody (mAb), Secondary antibody, could be used for ELISA, CLIA and so on.
Relevance IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
Product Properties
Antibody Type
secondary antibody
Clonality
Monoclonal antibodies
Isotype
IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Octylic acid-ammonium sulfate precipitation
Source
Hybridoma supernatant
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Storage: -20℃ for long term storage. Avoid repeated freeze / thaw cycles.
Images
Mouse Anti-Human IgG Antibody (Ab109057) - ELISA The Mouse Anti-Human IgG Antibody (Ab109057) conjugated with HRP (Horseradish Peroxidase) is designed for the specific detection and quantification of human IgG in various immunoassays, providing enhanced sensitivity and signal amplification for reliable results. The wells were coated with the anti-CD20 and BSA at 1.0 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Mouse Anti-Human IgG H&L (HRP) then added at different dilution ratio followed by incubation for 30 min.
Certificates
Certificate of Analysis(COA)
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ZJ23F0500134