Myc tag Mouse mAb - Primary antibody, specific to Myc, Mouse IgG1

    Application:
  • IF/ICC
  • IP
  • WB
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Species independent
  • Isotype: Mouse IgG1
  • Clone Number: 7D9-4C6-6F10
  • Conjugation: Unconjugated
Item Number
Ab116621
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab116621-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$49.90
Ab116621-50μl
50μl
In stock
$149.90
Ab116621-100μl
100μl
In stock
$209.90
Ab116621-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,599.90

mAb (7D9-4C6-6F10); Mouse anti Myc tag Antibody; WB, ICC, IF, IP; Unconjugated

View related series
Myc tag Tag

Basic Description

Product NameMyc tag Mouse mAb - Primary antibody, specific to Myc, Mouse IgG1
Synonymsc-myc tag antibody | Myc Epitope Tag antibody | avian myelocytomatosis viral oncogene homolog | BHLHE39 | bHLHe39MRTL | Class E basic helix-loop-helix protein 39 | cMyc | c-Myc | myc proto-oncogene protein | Myc | Myc2 | MYCC | myc-related translation/loc
Specifications & PurityExactAb™, Validated, High performance, 1.0 mg/mL
Host speciesMouse
SpecificityMyc
ImmunogenA synthetic peptide derived from Myc-Tag (EQKLISEEDL).
Positive ControlWB: 11654-myc lysates. ICC/IF: 293 cells transfected with a Myc tag protein. IP: Transfected 293 cell lysates with c-Myc tag fusion protein
ConjugationUnconjugated
GradeExactAb™, High Performance, Validated
Product Description

Mouse anti Myc tag Antibody, Monoclonal (7D9-4C6-6F10), could be used for WB, ICC, IF, IP and so on.
Application:
WB: 1/1000 - 1/10000
IF/ICC: 1/100 - 1/500
IP: 1/200
Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein鈥檚 biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

Product Properties

IsotypeMouse IgG1
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Myc tag Mouse mAb (Ab116621) - Western Blot
All lanes: Myc tag Mouse mAb (Ab116621) at 1/5000 dilution
Samples: Lysates at 5 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution

Predicted band size: 29 kDa
Observed band size: 32 kDa
Exposure time: 0.1 s

Myc tag Mouse mAb (Ab116621) - IP
Immunoprecipitation analysis of c-Myc Tag in
1: Untransfected HEK293 cell lysate
2: Transfected HEK293 cell lysate with c-Myc tag fusion protein
3: IP (transfected HEK293 + normal mouse IgG+Protein G agarose)
4: IP (transfected HEK293 + Myc tag Mouse mAb (Ab116621) + Protein G agarose)

Application

ApplicationDilution info
WB

1/1000 - 1/10000

IP

1/200

IF/ICC 1/100-1/500

">

1/100-1/500

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1101598Certificate of AnalysisNov 08, 2023 Ab116621
ZJ23F1101599Certificate of AnalysisNov 08, 2023 Ab116621

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