Myc tag Mouse mAb - Primary antibody, specific to Myc, Mouse IgG1
Synonyms
c-myc tag antibody | Myc Epitope Tag antibody | avian myelocytomatosis viral oncogene homolog | BHLHE39 | bHLHe39MRTL | Class E basic helix-loop-helix protein 39 | cMyc | c-Myc | myc proto-oncogene protein | Myc | Myc2 | MYCC | myc-related translation/loc
Specifications & Purity
ExactAb™, Validated, High performance, 1.0 mg/mL
Host species
Mouse
Specificity
Myc
Immunogen
A synthetic peptide derived from Myc-Tag (EQKLISEEDL).
Positive Control
WB: 11654-myc lysates. ICC/IF: 293 cells transfected with a Myc tag protein. IP: Transfected 293 cell lysates with c-Myc tag fusion protein
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, Validated
Product Description
Mouse anti Myc tag Antibody, Monoclonal (7D9-4C6-6F10), could be used for WB, ICC, IF, IP and so on. Application: WB: 1/1000 - 1/10000 IF/ICC: 1/100 - 1/500 IP: 1/200 Relevance Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein鈥檚 biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Product Properties
Isotype
Mouse IgG1
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Myc tag Mouse mAb (Ab116621) - Western Blot All lanes: Myc tag Mouse mAb (Ab116621) at 1/5000 dilution Samples: Lysates at 5 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution
Predicted band size: 29 kDa Observed band size: 32 kDa Exposure time: 0.1 s
Myc tag Mouse mAb (Ab116621) - IP Immunoprecipitation analysis of c-Myc Tag in 1: Untransfected HEK293 cell lysate 2: Transfected HEK293 cell lysate with c-Myc tag fusion protein 3: IP (transfected HEK293 + normal mouse IgG+Protein G agarose) 4: IP (transfected HEK293 + Myc tag Mouse mAb (Ab116621) + Protein G agarose)
Application
Application
Dilution info
WB
1/1000 - 1/10000
IP
1/200
IF/ICC
1/100-1/500
">
1/100-1/500
Certificates
Certificate of Analysis(COA)
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