aladdin Mycoplasma Prophylactic and EliminationReagent II can be used to prevent or eliminate mycoplasma that is commonly present in cell cultures. It not only eliminates mycoplasma that already exists in cells or culture medium, but also prevents mycoplasma contamination.Mycoplasma Prophylactic and EliminationReagent II mainly contains tetracycline and diterpenoid antibiotics that efficiently target mycoplasma. These two antibiotics inhibit protein synthesis through different mechanisms. Tetracycline antibiotics bind to the A sites of ribosomal 30S subunits, inhibiting peptide prolongation by preventing the binding of aminoyl-tRNA to this site. Diterpene antibiotics bind to ribosomal 50S subunit and inhibit peptidyl transferase activity. This product is only effective to mycoplasma and bacteria, and basically has no effect on the biological functions and growth of eukaryotic cells.The reagent stock is provided in solution. Add it directly to the culture liquid at 1
Precautions：
Aliquot prior to use. Avoid repeated freezing and thawing. Small amount of precipitation in the reagent after melting has no effect on its activity. Vortex to promote its dissolution.It is recommended to perform mycoplasma test in cultures periodically usingmycoplasma detection kits from , such as Myco-Lumi ™ luminescent Mycoplasma Detection Kit , Mycoplasma PCR Detection Kit or Mycoplasma Stain Kit .This reagent can effectively inhibit or eliminate many types of mycoplasma, but there is no guarantee that it will inhibit or eliminate any type of mycoplasma encountered during the laboratory operations. Resistance of mycoplasma to this reagent may be developed after long-term use. Under such circumstance, consider using other mycoplasma removal reagents from , such as Mycoplasma Prophylactic andEliminationReagent II , Mycoplasma Removal Reagent , or Mycoplasma Removal Reagent Plus .This product is toxic and irritating. Please handle it with care to avoid inhalation and direct contact with skin.This kit is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear lab coat and disposable gloves during the operation.
Instructions for Use：
1. Reagent preparation:Melt the reagent and dissolve completely. Take appropriate amount for instant use, aliquot the rest and keep at -20℃ for long-term storage.Note: Small amount of precipitation in the reagent after melting has no effect on its activity. Vortex to promote its dissolution.2. Prevention or inhibition of mycoplasma:Add the reagent to cell cultures at a dilution of 1:500. Mix well and culture cells under normal conditions. In general, it can effectively prevent mycoplasma contamination or inhibit mycoplasma proliferation.3. Elimination of mycoplasma:a. If cells are contaminated with mycoplasma, cultivate cells in culture medium supplemented with this reagent at a dilution of 1:100. Refresh the culture medium every 2-3 days. If mycoplasma is eliminated after 2-3 weeks, the culture medium can be replaced with normal culture medium without or with this reagent at 1:500 dilution. If mycoplasma is still detectable, consider using Luminescent Mycoplasma Detection Kit (for Low-Sensitivity Instrument) 20/100T C0298S/M Myco-Lumi™ Luminescent Mycoplasma Detection Kit (for High-Sensitivity Instrument) 20/100T C0299S Myco-Lumi™ Luminescent Mycoplasma Detection Postitive Control 20T C0301S PCR Mycoplasma Detection Kit 250T
