Basic Description

Synonyms	Bicine\|150-25-4\|N,N-Bis(2-hydroxyethyl)glycine\|Diethylolglycine\|Diethanol glycine\|Dihydroxyethylglycine\|Bicene\|N,N-Di(2-hydroxyethyl)glycine\|2-(bis(2-hydroxyethyl)amino)acetic acid\|N,N-Dihydroxyethylglycine\|N,N-Dihydroxyethyl glycine\|Glycine, N,N-bis(2-hy
Specifications & Purity	99%
Storage Temp	Room temperature
Shipped In	Normal
Product Description	Bicine is a zwitterionic amino acid buffer, active in the pH 7.6-9.0 range (pKa of 8.26 at 25°C). Recommended buffer for low temperature biochemical work. Bicine is used for the preparation of stable substrate solution for serum guanase determination. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described. Recommended buffer for low temperature biochemical work.Used in the preparation of stable substrate solution for serum guanase determination.Spacer in plasma protein fractionation with Sephadex? by isotachophoresis.

Names and Identifiers

IUPAC Name	2-[bis(2-hydroxyethyl)amino]acetic acid
INCHI	InChI=1S/C6H13NO4/c8-3-1-7(2-4-9)5-6(10)11/h8-9H,1-5H2,(H,10,11)
InChi Key	FSVCELGFZIQNCK-UHFFFAOYSA-N
Canonical SMILES	C(CO)N(CCO)CC(=O)O
Isomeric SMILES	C(CO)N(CCO)CC(=O)O
WGK Germany	1
RTECS	MB9700000
PubChem CID	8761
Molecular Weight	163.17
Beilstein	1769362
Reaxy-Rn	1769362

Certificates

Certificate of Analysis(COA)

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18 results found

Lot Number	Certificate Type	Date	Item
F2425094	Certificate of Analysis	Jun 29, 2024	B110905
F2425095	Certificate of Analysis	Jun 28, 2024	B110905
E2416407	Certificate of Analysis	Mar 18, 2024	B110905
E2416402	Certificate of Analysis	Mar 18, 2024	B110905
E2416406	Certificate of Analysis	Mar 18, 2024	B110905
A2429301	Certificate of Analysis	Jan 31, 2024	B110905
A2429302	Certificate of Analysis	Jan 31, 2024	B110905
L2322123	Certificate of Analysis	Dec 28, 2023	B110905
L2305025	Certificate of Analysis	Dec 07, 2023	B110905
I2311201	Certificate of Analysis	Sep 18, 2023	B110905
I2311202	Certificate of Analysis	Sep 18, 2023	B110905
I2311758	Certificate of Analysis	Sep 18, 2023	B110905
D23111115	Certificate of Analysis	Sep 12, 2022	B110905
I2206191	Certificate of Analysis	Sep 12, 2022	B110905
I2206201	Certificate of Analysis	Sep 12, 2022	B110905
A2215220	Certificate of Analysis	Jan 22, 2022	B110905
A2215219	Certificate of Analysis	Jan 22, 2022	B110905
A2215218	Certificate of Analysis	Jan 22, 2022	B110905

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Chemical and Physical Properties

Solubility	Solvent:water, Max Conc. mg/mL: 326.34, Max Conc. mM: 2000
Melt Point(°C)	190-192°C

Safety and Hazards(GHS)

WGK Germany	1
RTECS	MB9700000
Reaxy-Rn	1769362
Merck Index	1201

FAQs and Articles

References

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SKU	Size	Availability	Price
B110905-25g	25g	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$21.90
B110905-100g	100g	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$59.90
B110905-500g	500g	In stock	$242.90

N,N-Bis(2-hydroxyethyl)glycine - 99%, high purity , CAS No.150-25-4