D23061962Determine the necessary mass, volume, or concentration for preparing a solution.
Basic Description
| Storage Temp | Store at -20°C |
|---|---|
| Shipped In | Ice chest + Ice pads |
| Product Description | Features Ultrapure quality Sugar specificity: D-GalNAc Miogenic activity Non-reactive with human erythrocytes Lyophilized powder Product description Narcissus pseudonarcissus lectin (NPL) is isolated from daffodils. It exists as a dimeric protein with the molecular weight of 26 kDa composed by two subunits of 13 kDa each. The lectin has specificity toward alpha linked mannose. NPL is used in characterisation of early stages of apoptosis and is mitogenic for human lymphocytes. The lectin agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes . NPL lectin is supplied as a white lyophilized powder from a buffer containing 10 mM CH3COONH4. The purity is determined by SDS-PAGE, which generates one band at 13 kDa, corresponding to the two isomers (the lectin dissociates below pH 5.0 and above pH 9.0) Applications Mitogenic studies of human lymphocytes Agglutination studies Directions for use The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol. The solution may be reconstituted in this buffer to desired working concentration. Shipping and storage The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than five years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.
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