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Nesvacumab (anti-ANGPT2) (Ab170536) - ELISA
Immobilized human ANGPT2 at 2.0 μg/mL can bind Nesvacumab (anti-ANGPT2) (Ab170536) with the EC₅₀ of 19.65 ng/mL.
Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
SKU | Size | Availability | Price | Qty |
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Ab170536-100μg | 100μg | In stock | $49.90 |
Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
Product Name | Nesvacumab (anti-ANGPT2) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to ANGPT2, >95%, high purity, Human IgG1 |
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Synonyms | Anti-ANGPT2 Reference Antibody (nesvacumab) ; nesvacumab; AGPT 2 antibody; Agpt2 antibody; ANG 2 antibody; ANG-2 antibody; ANG2 antibody; Angiopoietin 2a antibody; Angiopoietin 2B antibody; Angiopoietin-2 antibody; Angiopoietin2 antibody; ANGP2_HUMAN anti |
Specifications & Purity | ExactAb™, Validated, Carrier Free, Low Endotoxin, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot |
Specificity | ANGPT2 |
Application | ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) |
Conjugation | Unconjugated |
Grade | Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated |
Action Type | INHIBITOR |
Mechanism of action | INHIBITOR of Angiopoietin-2 inhibitor |
Product Description | Nesvacumab (anti-ANGPT2) is a fully human immunoglobulin G1 (IgG1) monoclonal antibody that specifically binds and inactivates the Tie2 receptor ligand Angiopoietin-2 (Ang2) with high affinity, but shows no binding to Ang1. |
Antibody Type | Primary antibody |
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Clonality | Recombinant |
Isotype | Human IgG1 |
Light Chain Type | kappa |
SDS-PAGE | 25.9 kDa (Light Chain) & 50.1 kDa (Heavy Chain), under reducing conditions; 177.2 kDa, under non-reducing conditions. |
Purification Method | Protein A purified |
Purity | >95% |
Source | CHO supernatant |
Form | Liquid |
Concentration | Lot by Lot |
Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
Shipped In | Ice chest + Ice pads |
Stability And Storage | Store at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle. |
CAS | 1296818-77-3 |
Nesvacumab (anti-ANGPT2) (Ab170536) - ELISA
Immobilized human ANGPT2 at 2.0 μg/mL can bind Nesvacumab (anti-ANGPT2) (Ab170536) with the EC₅₀ of 19.65 ng/mL.
Nesvacumab (anti-ANGPT2) (Ab170536) - SEC
The purity of Nesvacumab (anti-ANGPT2) (Ab170536) is more than 95% verified by HPLC.
Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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IMGT/mAb-DB | 452 |
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Wikipedia | Nesvacumab |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
Lot Number | Certificate Type | Date | Item |
---|---|---|---|
ZJ24F0303687 | Certificate of Analysis | Mar 25, 2024 | Ab170536 |
ZJ24F0303686 | Certificate of Analysis | Mar 25, 2024 | Ab170536 |
ZJ24F0303685 | Certificate of Analysis | Mar 25, 2024 | Ab170536 |
1. Daly C, Eichten A, Castanaro C, Pasnikowski E, Adler A, Lalani AS, Papadopoulos N, Kyle AH, Minchinton AI, Yancopoulos GD et al.. (2013) Angiopoietin-2 functions as a Tie2 agonist in tumor models, where it limits the effects of VEGF inhibition.. Cancer Res, 73 (1): (108-18). [PMID:23149917] |
2. Gerald D, Chintharlapalli S, Augustin HG, Benjamin LE. (2013) Angiopoietin-2: an attractive target for improved antiangiogenic tumor therapy.. Cancer Res, 73 (6): (1649-57). [PMID:23467610] |
3. Regula JT, Lundh von Leithner P, Foxton R, Barathi VA, Cheung CM, Bo Tun SB, Wey YS, Iwata D, Dostalek M, Moelleken J et al.. (2016) Targeting key angiogenic pathways with a bispecific CrossMAb optimized for neovascular eye diseases.. EMBO Mol Med, 8 (11): (1265-1288). [PMID:27742718] |
4. Maisonpierre, P C PC and 13 more authors.. (1997) Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis.. Science (New York, N.Y.), (4): [PMID:9204896] |
5. Tanaka, S S and 5 more authors.. (1999) Biologic significance of angiopoietin-2 expression in human hepatocellular carcinoma.. The Journal of clinical investigation, [PMID:9927494] |
6. Kim, I I and 5 more authors.. (2000) Characterization and expression of a novel alternatively spliced human angiopoietin-2.. The Journal of biological chemistry, (16): [PMID:10766762] |
7. Fiedler, Ulrike U and 8 more authors.. (2003) Angiopoietin-1 and angiopoietin-2 share the same binding domains in the Tie-2 receptor involving the first Ig-like loop and the epidermal growth factor-like repeats.. The Journal of biological chemistry, (17): [PMID:12427764] |
8. Lee, Hyuek Jong HJ and 9 more authors.. (2004) Biological characterization of angiopoietin-3 and angiopoietin-4.. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, [PMID:15284220] |
9. Barton, William A WA, Tzvetkova, Dorothea D and Nikolov, Dimitar B DB.. (2005) Structure of the angiopoietin-2 receptor binding domain and identification of surfaces involved in Tie2 recognition.. Structure (London, England : 1993), [PMID:15893672] |
10. Nusbaum, Chad C and 75 more authors.. (2006) DNA sequence and analysis of human chromosome 8.. Nature, (19): [PMID:16421571] |
11. Barton, William A WA and 6 more authors.. (2006) Crystal structures of the Tie2 receptor ectodomain and the angiopoietin-2-Tie2 complex.. Nature structural & molecular biology, [PMID:16732286] |
12. Yacyshyn, Orisha K OK and 5 more authors.. (2009) Tyrosine phosphatase beta regulates angiopoietin-Tie2 signaling in human endothelial cells.. Angiogenesis, [PMID:19116766] |
13. Yuan, Hai Tao HT, Khankin, Eliyahu V EV, Karumanchi, S Ananth SA and Parikh, Samir M SM.. (2009) Angiopoietin 2 is a partial agonist/antagonist of Tie2 signaling in the endothelium.. Molecular and cellular biology, [PMID:19223473] |
14. Leppänen, Veli-Matti and 20 more authors.. (2020) Characterization of ANGPT2 mutations associated with primary lymphedema.. Science translational medicine, (9): [PMID:32908006] |