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Nesvacumab (anti-ANGPT2) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to ANGPT2, >95%, high purity, Human IgG1, INHIBITOR of Angiopoietin-2 inhibitor

  • Azide Free
  • Carrier Free
  • ExactAb™
  • Low Endotoxin
  • Recombinant
  • Validated
  • ≥95%(SDS-PAGE&SEC)
  • Lot by Lot
Features and benefits
  • Short Overview:

    Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated

  • Application: ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR)
  • Species reactivity(Reacts with): Cynomolgus monkey,Human,Mouse
  • Isotype: Human IgG1
    Application:
  • Animal Model
  • ELISA
  • Flow Cytometry
  • Functional Assay
Item Number
Ab170536
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab170536-100μg
100μg
In stock
$49.90

Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated

View related series
Accession#:O15123 ANGPT2 Gene ID:285

Basic Description

Product NameNesvacumab (anti-ANGPT2) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to ANGPT2, >95%, high purity, Human IgG1
SynonymsAnti-ANGPT2 Reference Antibody (nesvacumab) ; nesvacumab; AGPT 2 antibody; Agpt2 antibody; ANG 2 antibody; ANG-2 antibody; ANG2 antibody; Angiopoietin 2a antibody; Angiopoietin 2B antibody; Angiopoietin-2 antibody; Angiopoietin2 antibody; ANGP2_HUMAN anti
Specifications & PurityExactAb™, Validated, Carrier Free, Low Endotoxin, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot
SpecificityANGPT2
ApplicationELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR)
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated
Action TypeINHIBITOR
Mechanism of actionINHIBITOR of Angiopoietin-2 inhibitor
Product Description

Nesvacumab (anti-ANGPT2) is a fully human immunoglobulin G1 (IgG1) monoclonal antibody that specifically binds and inactivates the Tie2 receptor ligand Angiopoietin-2 (Ang2) with high affinity, but shows no binding to Ang1.
Purity
>95% (SDS-PAGE&SEC)
Endotoxin Level
< 1.0EU/mg

Product Properties

Antibody TypePrimary antibody
ClonalityRecombinant
IsotypeHuman IgG1
Light Chain Typekappa
SDS-PAGE25.9 kDa (Light Chain) & 50.1 kDa (Heavy Chain), under reducing conditions; 177.2 kDa, under non-reducing conditions.
Purification MethodProtein A purified
Purity>95%
SourceCHO supernatant
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -80°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle.
CAS1296818-77-3

Images

Nesvacumab (anti-ANGPT2) (Ab170536) - ELISA
Immobilized human ANGPT2 at 2.0 μg/mL can bind Nesvacumab (anti-ANGPT2) (Ab170536) with the EC₅₀ of 19.65 ng/mL.

Nesvacumab (anti-ANGPT2) (Ab170536) - SEC
The purity of Nesvacumab (anti-ANGPT2) (Ab170536) is more than 95% verified by HPLC.

Associated Targets

ANGPT2 Tclin Angiopoietin-2 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303687Certificate of AnalysisMar 25, 2024 Ab170536
ZJ24F0303686Certificate of AnalysisMar 25, 2024 Ab170536
ZJ24F0303685Certificate of AnalysisMar 25, 2024 Ab170536

Related Documents

References

1. Daly C, Eichten A, Castanaro C, Pasnikowski E, Adler A, Lalani AS, Papadopoulos N, Kyle AH, Minchinton AI, Yancopoulos GD et al..  (2013)  Angiopoietin-2 functions as a Tie2 agonist in tumor models, where it limits the effects of VEGF inhibition..  Cancer Res,  73  (1): (108-18).  [PMID:23149917]
2. Gerald D, Chintharlapalli S, Augustin HG, Benjamin LE.  (2013)  Angiopoietin-2: an attractive target for improved antiangiogenic tumor therapy..  Cancer Res,  73  (6): (1649-57).  [PMID:23467610]
3. Regula JT, Lundh von Leithner P, Foxton R, Barathi VA, Cheung CM, Bo Tun SB, Wey YS, Iwata D, Dostalek M, Moelleken J et al..  (2016)  Targeting key angiogenic pathways with a bispecific CrossMAb optimized for neovascular eye diseases..  EMBO Mol Med,  (11): (1265-1288).  [PMID:27742718]
4. Maisonpierre, P C PC and 13 more authors..  (1997)  Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis..  Science (New York, N.Y.),    (4):   [PMID:9204896]
5. Tanaka, S S and 5 more authors..  (1999)  Biologic significance of angiopoietin-2 expression in human hepatocellular carcinoma..  The Journal of clinical investigation,      [PMID:9927494]
6. Kim, I I and 5 more authors..  (2000)  Characterization and expression of a novel alternatively spliced human angiopoietin-2..  The Journal of biological chemistry,    (16):   [PMID:10766762]
7. Fiedler, Ulrike U and 8 more authors..  (2003)  Angiopoietin-1 and angiopoietin-2 share the same binding domains in the Tie-2 receptor involving the first Ig-like loop and the epidermal growth factor-like repeats..  The Journal of biological chemistry,    (17):   [PMID:12427764]
8. Lee, Hyuek Jong HJ and 9 more authors..  (2004)  Biological characterization of angiopoietin-3 and angiopoietin-4..  FASEB journal : official publication of the Federation of American Societies for Experimental Biology,      [PMID:15284220]
9. Barton, William A WA, Tzvetkova, Dorothea D and Nikolov, Dimitar B DB..  (2005)  Structure of the angiopoietin-2 receptor binding domain and identification of surfaces involved in Tie2 recognition..  Structure (London, England : 1993),      [PMID:15893672]
10. Nusbaum, Chad C and 75 more authors..  (2006)  DNA sequence and analysis of human chromosome 8..  Nature,    (19):   [PMID:16421571]
11. Barton, William A WA and 6 more authors..  (2006)  Crystal structures of the Tie2 receptor ectodomain and the angiopoietin-2-Tie2 complex..  Nature structural & molecular biology,      [PMID:16732286]
12. Yacyshyn, Orisha K OK and 5 more authors..  (2009)  Tyrosine phosphatase beta regulates angiopoietin-Tie2 signaling in human endothelial cells..  Angiogenesis,      [PMID:19116766]
13. Yuan, Hai Tao HT, Khankin, Eliyahu V EV, Karumanchi, S Ananth SA and Parikh, Samir M SM..  (2009)  Angiopoietin 2 is a partial agonist/antagonist of Tie2 signaling in the endothelium..  Molecular and cellular biology,      [PMID:19223473]
14. Leppänen, Veli-Matti and 20 more authors..  (2020)  Characterization of ANGPT2 mutations associated with primary lymphedema..  Science translational medicine,    (9):   [PMID:32908006]

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